336 0

Direct Observation of Interactions of Silk-Elastinlike Protein Polymer with Adenoviruses and Elastase

Title
Direct Observation of Interactions of Silk-Elastinlike Protein Polymer with Adenoviruses and Elastase
Author
윤채옥
Keywords
atomic force microscopy; silk-elastinlike protein polymers; adenovirus; elastase
Issue Date
2015-05
Publisher
AMER CHEMICAL SOC
Citation
MOLECULAR PHARMACEUTICS, v. 12, NO 5, Page. 1673-1679
Abstract
Silk-elastinlike protein polymer (SELP) hydrogels have been investigated for sustained local delivery of adenoviral gene carriers to solid tumors. These polymers degrade in the presence of proteases such as elastase. A detailed understanding of the interaction of SELPs with viruses and their degradation in the presence of elastase can provide useful information about mechanisms of sustained gene delivery from these systems. In this work, we investigated the interactions of SELPs with adenoviruses (Ads) and elastase using atomic force microscopy. We observed that viral particles interacted strongly with SELP networks formed by cross-linking of nanofibers. The presence of viruses contributed to enhanced network formation. Incubation of Ad with SELPs in the liquid state induced close packing of the viral colony. Morphological changes of SELP networks cleaved by enzymatic interaction with elastase were investigated. SELP-415K fiber networks were more responsive to temperature changes and were slowly degraded by elastases compared to SELP-47K, a SELP analogue with shorter elastin units in the monomer repeat. These studies provide insight into the influence of SELF structure on degradation and potential mechanisms of increased viral stability.
URI
http://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.5b00075http://hdl.handle.net/20.500.11754/24861
ISSN
1543-8384
DOI
10.1021/acs.molpharmaceut.5b00075
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE