Rh D blood group conversion using transcription activator-like effector nucleases
- Title
- Rh D blood group conversion using transcription activator-like effector nucleases
- Author
- 백은정
- Keywords
- ZINC-FINGER NUCLEASES; INDUCED MUTATIONS; SURROGATE REPORTERS; TERMINATION CODON; GENE MODIFICATION; RNA SURVEILLANCE; CELLS; ENRICHMENT; GENOME; GALACTOSIDASE
- Issue Date
- 2015-05
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- NATURE COMMUNICATIONS, v. 6, Page. 1-12
- Abstract
- Group O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported. The Rh D blood group is determined by the RHD gene, which encodes a 12-transmembrane domain protein. Here we convert Rh D-positive erythroid progenitor cells into D-negative cells using RHD-targeting transcription activator-like effector nucleases (TALENs). After transfection of TALEN-encoding plasmids, RHD-knockout clones are obtained. Erythroid-lineage cells differentiated from these knockout erythroid progenitor cells do not agglutinate in the presence of anti-D reagents and do not express D antigen, as assessed using flow cytometry. Our programmable nuclease-induced blood group conversion opens new avenues for compatible donor cell generation in transfusion medicine.
- URI
- http://www.nature.com/articles/ncomms8451http://hdl.handle.net/20.500.11754/24749
- ISSN
- 2041-1723
- DOI
- 10.1038/ncomms8451
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
- Files in This Item:
- Rh D blood group conversion using transcription activator-like effector nucleases.pdfDownload
- Export
- RIS (EndNote)
- XLS (Excel)
- XML