230 0

Endothelial-to-mesenchymal transition induced by Wnt 3a in keloid pathogenesis

Title
Endothelial-to-mesenchymal transition induced by Wnt 3a in keloid pathogenesis
Author
윤채옥
Keywords
GROWTH-FACTOR-BETA; HYPERTROPHIC SCARS; IN-VIVO; FIBROSIS; FIBROBLASTS; MYOFIBROBLAST; MECHANISMS; EXPRESSION; CELLS; SKIN
Issue Date
2015-05
Publisher
WILEY-BLACKWELL
Citation
WOUND REPAIR AND REGENERATION, v. 23, NO 3, Page. 435-442
Abstract
Endothelial-to-mesenchymal transition is a phenotypic conversion characterized by down-regulation of vascular endothelial markers and the acquisition of a mesenchymal phenotype. We hypothesized that keloid fibroblasts are of endothelial origin and that endothelial-to-mesenchymal transition substantially contributes to collagen accumulation during the development and progression of keloids. Wingless protein (Wnt-3a) protein expression was examined using immunohistochemistry in keloid tissues. Human dermal microvascular endothelial cells (HDMECs) were treated with Wnt-3a. mRNA and protein expression of endothelial (vascular endothelial cadherin) and mesenchymal (vimentin, snail family transcription factor [slug], and -smooth muscle actin) cell markers were measured using real-time RT-PCR and immunocytochemistry, respectively. Additionally, coexpression of CD31 (cluster of differentiation 31), and endothelial cell marker, and vimentin in the vascular endothelium of keloid tissues was examined using immunofluorescence. Wnt-3a overexpression was observed in human keloid tissues. Wnt-3a treatment significantly reduced vascular endothelial cadherin mRNA expression and induced vimentin and slug mRNA expression in HDMECs. HDMECs became spindle-shaped and exhibited reduced expression of CD31 and increased expression of vimentin, slug, and -smooth muscle actin. Moreover, coexpression of CD31 and vimentin was observed in the dermal vascular endothelium of keloid tissues from two patients with clinically active keloids. In conclusion, transient conversion of HDMECs to a mesenchymal phenotype may contribute to dermal fibrosis of keloid and hypertrophic scars.
URI
http://onlinelibrary.wiley.com/doi/10.1111/wrr.12300/abstracthttp://hdl.handle.net/20.500.11754/24465
ISSN
1067-1927; 1524-475X
DOI
10.1111/wrr.12300
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE