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Effect of the Mechanical Properties of Cell-Interactive Hydrogels on a Control of Cell Phenotype

Title
Effect of the Mechanical Properties of Cell-Interactive Hydrogels on a Control of Cell Phenotype
Author
이근용
Keywords
hydrogel; stiffness; alginate; adhesion; proliferation
Issue Date
2015-05
Publisher
POLYMER SOC KOREA
Citation
POLYMER-KOREA, v. 39, NO 3, Page. 412-417
Abstract
A critical element in tissue engineering approaches is a control of the mechanical properties of polymer scaffolds to regulate cell phenotype, which may lead to clinically successful tissue regeneration. In this study, we hypothesized that gel stiffness could be a key factor to manipulate adhesion and proliferation of different types of cells. RGD-modified alginate gels with various mechanical properties were prepared and used as a substrate for MC3T3-E1 and H9C2 cells. Adhesion and growth rate of MC3T3-E1 cells in vitro were increased in parallel with an increase of gel stiffness. In contrast, those of H9C2 cells were decreased. This approach to control the mechanical properties of polymer scaffolds depending on the cell types may find useful applications in the tissue engineering. 조직공학에 있어서 고분자 지지체의 물성은 세포의 부착, 이동, 성장 및 분화에 영향을 미치는 중요한 요소 중 하나이다. 이 논문에서는 다양한 강성을 가지는 세포 친화적인 알긴산 하이드로젤을 제조하고 골모세포(MC3T3-E1)와 심근세포(H9C2)를 2차원 배양한 후, 각 세포의 부착 및 성장을 연구하였다. 골조직에서 유래한 C3T3-E1세포는 하이드로젤의 강성도가 증가함에 따라 성장이 촉진되었지만 근육조직 유래의 H9C2세포는 오히려 감소하였다. 재생하고자 하는 조직의 종류에 따라 지지체의 기계적인 물성을 변화시켜서 세포의 부착 및 성장을 제어하는 것은 조직공학적으로 조직 및 장기를 개발하는 데 있어서 중요한 역항을 할 것이다.
URI
http://koreascience.or.kr/article/ArticleFullRecord.jsp?cn=GBJHCY_2015_v39n3_412http://hdl.handle.net/20.500.11754/24366
ISSN
0379-153X; 2234-8077
DOI
10.7317/pk.2015.39.3.412
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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