Fast Protein Liquid Chromatography를 이용한 황색포도상구균 장독소 B분리
- Title
- Fast Protein Liquid Chromatography를 이용한 황색포도상구균 장독소 B분리
- Other Titles
- Separation of Staphylococcal Enterotoxin B by Fast Protein Liquid Chromatography
- Author
- 엄애선
- Keywords
- staphylococcal enterotoxin B; SEB; fast protein liquid chromatography; FPLC; separation; detection; purification
- Issue Date
- 2015-05
- Publisher
- 한국산업식품공학회
- Citation
- 산업식품공학, v. 19, NO 2, Page. 111-116
- Abstract
- The aim of this study is to develop a method to separate and purify the Staphylococcal enterotoxin B (SEB). The method was based on fast protein liquid chromatography (FPLC) along with gel filtration (GF) after cation exchange chromatography (CIEX) treatment. Various columns such as CM sepharose HP, Cellufine C-500, WorkBeads 40S for CIEX, and GF on Sephacryl S-100 for GF were compared in terms of separation efficiency. Consequently, Work- Beads 40S along with Sephacryl S-100 produced best separation performance. Separated SEB with developed method was verified with ELISA assay. Purified SEB with the method can be used to manufacture rapid detection kit or antibodies.
- URI
- http://kiss.kstudy.com/journal/thesis_name.asp?tname=kiss2002&key=3321517http://hdl.handle.net/20.500.11754/24326
- ISSN
- 1226-4768; 2288-1247
- DOI
- 10.13050/foodengprog.2015.19.2.111
- Appears in Collections:
- COLLEGE OF HUMAN ECOLOGY[S](생활과학대학) > FOOD & NUTRITION(식품영양학과) > Articles
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