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Fast Protein Liquid Chromatography를 이용한 황색포도상구균 장독소 B분리

Title
Fast Protein Liquid Chromatography를 이용한 황색포도상구균 장독소 B분리
Other Titles
Separation of Staphylococcal Enterotoxin B by Fast Protein Liquid Chromatography
Author
엄애선
Keywords
staphylococcal enterotoxin B; SEB; fast protein liquid chromatography; FPLC; separation; detection; purification
Issue Date
2015-05
Publisher
한국산업식품공학회
Citation
산업식품공학, v. 19, NO 2, Page. 111-116
Abstract
The aim of this study is to develop a method to separate and purify the Staphylococcal enterotoxin B (SEB). The method was based on fast protein liquid chromatography (FPLC) along with gel filtration (GF) after cation exchange chromatography (CIEX) treatment. Various columns such as CM sepharose HP, Cellufine C-500, WorkBeads 40S for CIEX, and GF on Sephacryl S-100 for GF were compared in terms of separation efficiency. Consequently, Work- Beads 40S along with Sephacryl S-100 produced best separation performance. Separated SEB with developed method was verified with ELISA assay. Purified SEB with the method can be used to manufacture rapid detection kit or antibodies.
URI
http://kiss.kstudy.com/journal/thesis_name.asp?tname=kiss2002&key=3321517http://hdl.handle.net/20.500.11754/24326
ISSN
1226-4768; 2288-1247
DOI
10.13050/foodengprog.2015.19.2.111
Appears in Collections:
COLLEGE OF HUMAN ECOLOGY[S](생활과학대학) > FOOD & NUTRITION(식품영양학과) > Articles
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