Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 엄애선 | - |
dc.date.accessioned | 2016-11-02T00:31:24Z | - |
dc.date.available | 2016-11-02T00:31:24Z | - |
dc.date.issued | 2015-04 | - |
dc.identifier.citation | FISHERIES SCIENCE, v. 81, NO 3, Page. 495-504 | en_US |
dc.identifier.issn | 0919-9268 | - |
dc.identifier.issn | 1444-2906 | - |
dc.identifier.uri | http://link.springer.com/article/10.1007%2Fs12562-015-0868-5 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/24058 | - |
dc.description.abstract | Insulin-like peptide (ILP) is an important hormone that controls a wide array of functions including glucose or trehalose levels in body fluids in invertebrates. However, the presence and role of ILP in copepods is unclear. To better understand the functional role of ILP in marine copepods, we isolated insulin-like peptide 1 (Tj-ILP1) from the copepod Tigriopus japonicus, characterized its conserved motifs, and measured its transcriptional modulation in response to different food sources. The open reading frame of Tj-ILP1 cDNA was 525 bp in length, encoding 174 amino acid residues. Multiple alignments revealed that six conserved cysteine residues in the ILP peptide domain were conserved for maintaining a tertiary structure. The Tj-ILP gene showed high expression levels in adult males, suggesting an association with sexual differentiation and male morphogenesis in T. japonicus. Additionally, to examine the role of Tj-ILP in dietary metabolism, we provided two food supplies (Chlorella vulgaris and Tetraselmis suecica) to copepods and measured dose- and time-dependent expression of Tj-ILP1 mRNA in response to different carbohydrate sources. Tj-ILP1 transcription activity was significantly elevated in response to both algal food supplies, indicating that the Tj-ILP1 gene is inducible with different food sources. | en_US |
dc.description.sponsorship | We thank Prof. Hans-U. Dahms for his comments on the manuscript, and we also thank the two anonymous reviewers and Prof. Shigeru Okada for their valuable comments. This work was supported by a grant from the Korea Polar Ocean Development program (K-POD; project no. PM13030) funded by the Ministry of Oceans and Fisheries, Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | SPRINGER JAPAN KK | en_US |
dc.subject | Copepod | en_US |
dc.subject | Tigriopus japonicus | en_US |
dc.subject | Insulin-like peptide 1 | en_US |
dc.subject | Food sources | en_US |
dc.title | Identification of insulin-like peptide 1 (ILP1) gene and its expression in response to different food sources in the intertidal copepod Tigriopus japonicus | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 81 | - |
dc.identifier.doi | 10.1007/s12562-015-0868-5 | - |
dc.relation.page | 495-504 | - |
dc.relation.journal | FISHERIES SCIENCE | - |
dc.contributor.googleauthor | Lee, Min Chul | - |
dc.contributor.googleauthor | Won, Eun-Ji | - |
dc.contributor.googleauthor | Eun-Ji, Seung-Hwi | - |
dc.contributor.googleauthor | Hwang, Dae-Sik | - |
dc.contributor.googleauthor | Kim, Hui-Su | - |
dc.contributor.googleauthor | Han, Jeonghoon | - |
dc.contributor.googleauthor | Rhee, Jae-Sung | - |
dc.contributor.googleauthor | Om, Ae-Son | - |
dc.contributor.googleauthor | Lee, Jae-Seong | - |
dc.relation.code | 2015005562 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF HUMAN ECOLOGY[S] | - |
dc.sector.department | DEPARTMENT OF FOOD & NUTRITION | - |
dc.identifier.pid | aesonom | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.