Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 정일엽 | - |
dc.date.accessioned | 2016-10-31T05:14:48Z | - |
dc.date.available | 2016-10-31T05:14:48Z | - |
dc.date.issued | 2015-04 | - |
dc.identifier.citation | ELECTROPHORESIS, v. 36, Page. 994-1001 | en_US |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.issn | 1522-2683 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/10.1002/elps.201400465/abstract | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/24021 | - |
dc.description.abstract | We developed the photo-crosslinkable hydrogel-based 3D microfluidic device to culture neural stem cells (NSCs) and tumors. The photo-crosslinkable gelatin methacrylate (GelMA) polymer was used as a physical barrier in the microfluidic device and collagen type I gel was employed to culture NSCs in a 3D manner. We demonstrated that the pore size was inversely proportional to concentrations of GelMA hydrogels, showing the pore sizes of 5 and 25 w/v% GelMA hydrogels were 34 and 4 m, respectively. It also revealed that the morphology of pores in 5 w/v% GelMA hydrogels was elliptical shape, whereas we observed circular-shaped pores in 25 w/v% GelMA hydrogels. To culture NSCs and tumors in the 3D microfluidic device, we investigated the molecular diffusion properties across GelMA hydrogels, indicating that 25 w/v% GelMA hydrogels inhibited the molecular diffusion for 6 days in the 3D microfluidic device. In contrast, the chemicals were diffused in 5 w/v% GelMA hydrogels. Finally, we cultured NSCs and tumors in the hydrogel-based 3D microfluidic device, showing that 53-75% NSCs differentiated into neurons, while tumors were cultured in the collagen gels. Therefore, this photo-crosslinkable hydrogel-based 3D microfluidic culture device could be a potentially powerful tool for regenerative tissue engineering applications. | en_US |
dc.description.sponsorship | This research was supported by BioNano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as Global Frontier Project (grant number H-GUARD_2014M3A6B2060503), Republic of Korea. This work was also supported by Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP) (grant number 2013K1A4A3055268). This work was also supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health & Welfare, Republic of Korea (Grant number HI14C3347). | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY-BLACKWELL | en_US |
dc.subject | Hydrogel | en_US |
dc.subject | Microfluidic device | en_US |
dc.subject | Stem cell | en_US |
dc.title | Photo-crosslinkable hydrogel-based 3D microfluidic culture device | en_US |
dc.type | Article | en_US |
dc.relation.volume | 36 | - |
dc.identifier.doi | 10.1002/elps.201400465 | - |
dc.relation.page | 994-1001 | - |
dc.relation.journal | ELECTROPHORESIS | - |
dc.contributor.googleauthor | Lee, Youlee | - |
dc.contributor.googleauthor | Lee, Jong Min | - |
dc.contributor.googleauthor | Bae, Pan-Kee | - |
dc.contributor.googleauthor | Chung, Il Yup | - |
dc.contributor.googleauthor | Chung, Bong Hyun | - |
dc.contributor.googleauthor | Chung, Bong Geun | - |
dc.relation.code | 2015000387 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | iychu | - |
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