Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신원선 | - |
dc.date.accessioned | 2016-10-17T01:46:11Z | - |
dc.date.available | 2016-10-17T01:46:11Z | - |
dc.date.issued | 2015-04 | - |
dc.identifier.citation | FOOD CHEMISTRY, v. 173, Page. 1-6 | en_US |
dc.identifier.issn | 0308-8146 | - |
dc.identifier.issn | 1873-7072 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0308814614015568 | - |
dc.description.abstract | Bovine serum albumin (BSA)-fucoidan conjugates were prepared by the Maillard reaction (60 degrees C and 79% relative humidity for 96 h), and were then identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and size-exclusion chromatography (SEC). Molecular characteristics of the BSA-fucoidan conjugates were investigated, using atomic force microscopy (AFM), dynamic light scattering (DLS), fluorescence spectroscopy, and circular dichroism spectroscopy. SDS-PAGE patterns provided evidence for the covalent bonding between BSA and fucoidan. SEC profiles showed that about 1.5-2.0 tool of fucoidan were covalently linked to 1 mol of BSA, resulting in high-molecular-weight compositions (conjugates). AFM images and DLS results indicated that most particles in the conjugates were nanostructured and more spherical than those of a regular BSA-fucoidan mixture. The fluorescence intensity and maximum emission wavelength of the conjugates together revealed that the BSA molecules had converted from an ordered conformation into a partially folded molten globule state. (C) 2014 Elsevier Ltd. All rights reserved. | en_US |
dc.description.sponsorship | This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. 2010-0025825). We greatly thank Professor Sang Guan You at Gangneung-Wonju National University for providing us with the light scattering equipment and for useful discussions. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | Bovine serum albumin | en_US |
dc.subject | Fucoidan | en_US |
dc.subject | Maillard reaction | en_US |
dc.subject | Molten globule state | en_US |
dc.title | Characterisation of bovine serum albumin-fucoidan conjugates prepared via the Maillard reaction | en_US |
dc.type | Article | en_US |
dc.relation.volume | 173 | - |
dc.identifier.doi | 10.1016/j.foodchem.2014.09.167 | - |
dc.relation.page | 1-6 | - |
dc.relation.journal | FOOD CHEMISTRY | - |
dc.contributor.googleauthor | Kim, Do-Yeong | - |
dc.contributor.googleauthor | Shin, Weon-Sun | - |
dc.relation.code | 2015002513 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF HUMAN ECOLOGY[S] | - |
dc.sector.department | DEPARTMENT OF FOOD & NUTRITION | - |
dc.identifier.pid | hime | - |
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