Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hyunsook Kim | - |
dc.date.accessioned | 2016-10-10T05:15:26Z | - |
dc.date.available | 2016-10-10T05:15:26Z | - |
dc.date.issued | 2015-04 | - |
dc.identifier.citation | JOURNAL OF FOOD PROTECTION, v. 78, NO 4, Page. 855-858 | en_US |
dc.identifier.issn | 0362-028X | - |
dc.identifier.issn | 1944-9097 | - |
dc.identifier.uri | http://search.proquest.com/docview/1668018751?accountid=11283 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/23695 | - |
dc.description.abstract | Lactobacillus kefiranofaciens is an indicator microorganism for kefir and a key factor in kefir grain formation and kefiran production. We designed a novel real-time PCR primer and probe set, LKF_KU504, for the rapid detection of L. kefiranofaciens. In inclusivity and exclusivity tests, only 14 L. kefiranofaciens strains were positive among 61 microorganisms, indicating 100% sensitivity and specificity. The LKF_KU504 set also differentiated kefir milk from 30 commercial nonkefir yogurts. The levels of L. kefiranofaciens in kefir grain and kefir milk were significantly different, indicating L. kefiranofaciens was more concentrated in kefir grain than in kefir milk | en_US |
dc.description.sponsorship | This work was supported by a National Research Foundation of Korea grant funded by the Korean government (MEST; nr 2012R1A2A2A01015344). D.-H. Kim, J.-W. Chon, H.-S. Kim, and J.-H. Yim were also partly supported by the Brain Korean 21 project from the Ministry of Education and Human Resources Development. | en_US |
dc.language.iso | en | en_US |
dc.publisher | INT ASSOC FOOD PROTECTION | en_US |
dc.subject | LACTIC-ACID BACTERIA | en_US |
dc.subject | CULTURE-INDEPENDENT METHODS | en_US |
dc.subject | TIBET KEFIR | en_US |
dc.subject | M1 | en_US |
dc.title | Rapid Detection of Lactobacillus kefiranofaciens in Kefir Grain and Kefir Milk Using Newly Developed Real-Time PCR | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 78 | - |
dc.identifier.doi | 10.4315/0362-028X.JFP-14-329 | - |
dc.relation.page | 855-858 | - |
dc.relation.journal | JOURNAL OF FOOD PROTECTION | - |
dc.contributor.googleauthor | Kim, Dong-Hyeon | - |
dc.contributor.googleauthor | Chon, Jung-Whan | - |
dc.contributor.googleauthor | Kim, Hong-Seok | - |
dc.contributor.googleauthor | Yim, Jin-Hyeok | - |
dc.contributor.googleauthor | Kim, Hyunsook | - |
dc.contributor.googleauthor | Seo, Kun-Ho | - |
dc.relation.code | 2015002175 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF HUMAN ECOLOGY[S] | - |
dc.sector.department | DEPARTMENT OF FOOD & NUTRITION | - |
dc.identifier.pid | hyunsk15 | - |
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