Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2016-07-14T02:28:49Z | - |
dc.date.available | 2016-07-14T02:28:49Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.citation | ONCOTARGET, v. 6, NO 6, Page. 4051-4065 | en_US |
dc.identifier.isbn | 1949-2553 | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414172/#? | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/22009 | - |
dc.description.abstract | c-Met, a cognate receptor tyrosine kinase of hepatocyte growth factor, is overexpressed and/or mutated in number of tumors. Therefore, abrogation of c-Met signaling may serve as potential therapeutic targets. In this study, we generated Ads expressing single shRNA specific to c-Met (shMet) (dl/shMet4 and dl/shMet5) or dual shRNAs specific to c-Met (dl/shMet4+5); and examined the therapeutic potential of these newly engineered Ads in targeting c-Met, and delineated their mechanism of action in vitro and in vivo. Ads expressing shMet induced knock-down in c-Met, and phenotypically resulted in autophagy-like features including appearance of membranousvacuoles, formation of acidic vesicular organelles, and cleavage and recruitment of microtubule-associated protein1 light chain 3 to autophagosomes. Ads expressing shMet also suppressed Akt phosphorylation and increased number of senescence-related gene products including SM22, TGase II, and PAI-1. These changes resulted in inhibition of cell proliferation and G2/M arrest of U343 cells. In vivo, intratumoral injection with dl/shMet4+5 resulted in a significant reduction of tumor growth with corresponding increasing overall survival. Histopathological analysis of these treated tumors revealed that Atg5 was highly up-regulated, indicating the therapeutic induction of autophagy. In sum, these results reveal that autophagic cell death induced by shMet-expressing Ads provide a novel strategy for targeting c-Met-expressing tumors through non-apoptotic mechanism of cell death. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | IMPACT JOURNALS LLC | en_US |
dc.subject | adenovirus | en_US |
dc.subject | autophagic cell death | en_US |
dc.subject | cancer gene therapy | en_US |
dc.subject | c-Met | en_US |
dc.subject | short hairpin RNA (shRNA) | en_US |
dc.title | Adenovirus expressing dual c-Met-specific shRNA exhibits potent antitumor effect through autophagic cell death accompanied by senescence-like phenotypes in glioblastoma cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 6 | - |
dc.relation.volume | 6 | - |
dc.relation.page | 4051-4065 | - |
dc.relation.journal | ONCOTARGET | - |
dc.contributor.googleauthor | Lee, Jung-Sun | - |
dc.contributor.googleauthor | Oh, Eonju | - |
dc.contributor.googleauthor | Yoo, Ji Young | - |
dc.contributor.googleauthor | Choi, Kyeong Sook | - |
dc.contributor.googleauthor | Yoon, Mi Jin | - |
dc.contributor.googleauthor | Yun, Chae-Ok | - |
dc.relation.code | 2015011641 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | chaeok | - |
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