One step in-situ synthesis of amine functionalized graphene for immunosensing of cardiac marker cTnI
- Title
- One step in-situ synthesis of amine functionalized graphene for immunosensing of cardiac marker cTnI
- Author
- Akash Deep
- Keywords
- Graphene; 2-Aminobenzyl amine; Biomarker; Cardiac TroponinI; Immunosensor
- Issue Date
- 2015-01
- Publisher
- Elsevier
- Citation
- Biosensors and Bioelectronics, v. 66, Page. 129-135
- Abstract
- 2-Aminobenzyl amine (2-ABA) functionalized graphene is proposed for the ultrasensitive immunosensing of Cardiac Troponin I (cTnI). 2-ABA was electrochemically polymerized on the graphene decorated interdigitated electrode to obtain the amine functionalized graphene (f-GN). The f-GN electrode was then modified with monoclonal anti-cTnI antibodies via Schiff reaction based chemistry. Detailed characteristics of the processes involved and the finally developed antibody conjugated f-GN interdigitated electrode have been studied. The above micro-device was used in a drain source configuration for the sensing of cTnI. A wide dynamic linear range of antigen detection (0.01–1 ng/mL) is achieved with the limit of detection of 0.01 ng/mL. The utility of the proposed sensing technique is demonstrated by successfully testing the antigen concentration in spiked serum samples.
- URI
- http://hdl.handle.net/20.500.11754/21747http://www.sciencedirect.com/science/article/pii/S0956566314008720
- ISSN
- 0956-5663
- DOI
- 10.1016/j.bios.2014.10.072
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > CIVIL AND ENVIRONMENTAL ENGINEERING(건설환경공학과) > Articles
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