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dc.contributor.author이수재-
dc.date.accessioned2016-05-25T07:54:47Z-
dc.date.available2016-05-25T07:54:47Z-
dc.date.issued2015-01-
dc.identifier.citationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v. 167, Page. 15-23en_US
dc.identifier.issn1532-0456-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/21351-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S1532045614001082-
dc.description.abstractUltraviolet B (UV-B) radiation presents an environmental hazard to aquatic organisms. To understand the molecular responses of the intertidal copepod Tigriopus japonicus to UV-B radiation, we measured the acute toxicity response to 96 h of UV-B radiation, and we also assessed the intracellular reactive oxygen species (ROS) levels, glutathione (GSH) content, and antioxidant enzyme (GST, GR, GPx, and SOD) activities after 24 h of exposure to UV-B with LD50 and half LD50 values. Also, expression patterns of p53 and hsp gene families with phosphorylation of p38 MAPK were investigated in UV-B-exposed copepods. We found that the ROS level, GSH content, and antioxidant enzyme activity levels were increased with the transcriptional upregulation of antioxidant-related genes, indicating that UV-B induces oxidative stress by generating ROS and stimulating antioxidant enzymatic activity as a defense mechanism. Additionally, we found that p53 expression was significantly increased after UV-B irradiation due to increases in the phosphorylation of the stress-responsive p38 MAPK, indicating that UV-B may be responsible for inducing DNA damage in T. japonicus. Of the hsp family genes, transcriptional levels of hsp20, hsp20.7, hsp70, and hsp90 were elevated in response to a low dose of UV-B radiation (9 kJ m(-2)), suggesting that these hsp genes may be involved in cellular protection against UV-B radiation. In this paper, we performed a pathway-oriented mechanistic analysis in response to UV-B radiation, and this analysis provides a better understanding of the effects of UV-B in the intertidal benthic copepod T. japonicus. (C) 2014 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipWe thank two anonymous reviewers for their constructive comments on the previous manuscript. This work was supported by a grant from the National Research Foundation (2012R1A2A2A02012617) to Jae-Seong Lee.-
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE INCen_US
dc.subjectp53en_US
dc.subjectCopepod; UV-Ben_US
dc.subjectTigriopus japonicusen_US
dc.subjectOxidative stressen_US
dc.subjectp38 MAPKen_US
dc.subjectHeat shock proteinen_US
dc.titleUV-B radiation-induced oxidative stress and p38 signaling pathway involvement in the benthic copepod Tigriopus japonicusen_US
dc.typeArticleen_US
dc.relation.volume167-
dc.identifier.doi10.1016/j.cbpc.2014.08.003-
dc.relation.page15-23-
dc.relation.journalCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY-
dc.contributor.googleauthorKim, Bo-Mi-
dc.contributor.googleauthorRhee, Jae-Sung-
dc.contributor.googleauthorLee, Kyun-Woo-
dc.contributor.googleauthorShin, Kyung-Hoon-
dc.contributor.googleauthorKim, Min-Jung-
dc.contributor.googleauthorLee, Young-Mi-
dc.contributor.googleauthorLee, Su-Jae-
dc.contributor.googleauthorLee, Jae-Seong-
dc.relation.code2015002029-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF LIFE SCIENCE-
dc.identifier.pidsj0420-
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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