Chemical constituents of the Ajuga multiflora bunge and their protective effects on dexamethasone-induced muscle atrophy in C2C12 myotubes
- Title
- Chemical constituents of the Ajuga multiflora bunge and their protective effects on dexamethasone-induced muscle atrophy in C2C12 myotubes
- Author
- 김재용
- Keywords
- Ajuga multiflora Bunge; 29-hydroxyprecyasterone; dexamethasone; muscle atrophy; C2C12 myotube
- Issue Date
- 2022-08-23
- Publisher
- TAYLOR & FRANCIS LTD
- Citation
- NATURAL PRODUCT RESEARCH, v. 37, no 12, page. 1978-1985
- Abstract
- Ajuga multiflora Bunge is a perennial ornamental herb and has been used for the treatment of fever in Korean folk medicine. In the course of searching for protective agents associated with the potential of A. multiflora against dexamethsone (DEX)-induced muscle atrophy, a new phytoecdysteroid, 29-hydroxyprecyasterone (1), together with four known compounds (2-5), were isolated from A. multiflora. The structures of the compounds were determined by spectroscopic analyses, including 1D-, 2D-NMR and HR-MS interpretation. To elucidate the effects of obtained compounds on DEX-induced muscle atrophy, the myotubes diameter, myosin heavy chain (MyHC) positive area, and fusion index were evaluated by immunofluorescence staining. Overall, each compound treatment effectively prevented the atrophic myotubes through an increase of MyHC-positive myotubes and the number of nuclei. Particularly, the measurement of myotube diameter showed that compounds 1 and 5 treatment significantly alleviated the myotube thickness.
- URI
- https://www.tandfonline.com/doi/full/10.1080/14786419.2022.2115491https://repository.hanyang.ac.kr/handle/20.500.11754/191610
- ISSN
- 1478-6427
- DOI
- VOL. 37, NO. 12;1978–1985
- Appears in Collections:
- RESEARCH INSTITUTE[E](부설연구소) > INSTITUTE OF PHARMACEUTICAL SCIENCE AND TECHNOLOGY(약학기술연구소) > Articles
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