Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 임동우 | - |
dc.date.accessioned | 2024-05-23T00:32:58Z | - |
dc.date.available | 2024-05-23T00:32:58Z | - |
dc.date.issued | 2023-06-29 | - |
dc.identifier.citation | ACS APPLIED MATERIALS & INTERFACES, v. 15, NO 27, Page. 32201-32214 | en_US |
dc.identifier.issn | 1944-8252 | en_US |
dc.identifier.issn | 1944-8244 | en_US |
dc.identifier.uri | https://information.hanyang.ac.kr/#/eds/detail?an=edselc.2-52.0-85164625617&dbId=edselc | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/190379 | - |
dc.description.abstract | Genetically engineered fusion polypeptideshave beeninvestigatedto introduce unique bio-functionality and improve some therapeuticactivity for anti-angiogenesis. We report herein that stimuli-responsive,vascular endothelial growth factor receptor 1 (VEGFR1) targeting fusionpolypeptides composed of a VEGFR1 (fms-like tyrosine kinase-1 (Flt1))antagonist, an anti-Flt1 peptide, and a thermally responsive elastin-basedpolypeptide (EBP) were rationally designed at the genetic level, biosynthesized,and purified by inverse transition cycling to develop potential anti-angiogenicfusion polypeptides to treat neovascular diseases. A series of hydrophilicEBPs with different block lengths were fused with an anti-Flt1 peptide,forming anti-Flt1-EBPs, and the effect of EBP block length on theirphysicochemical properties was examined. While the anti-Flt1 peptidedecreased phase-transition temperatures of anti-Flt1-EBPs, comparedwith EBP blocks, anti-Flt1-EBPs were soluble under physiological conditions.The anti-Flt1-EBPs dose dependently inhibited the binding of VEGFR1against vascular endothelial growth factor (VEGF) as well as tube-likenetwork formation of human umbilical vein endothelial cells underVEGF-triggered angiogenesis in vitro because of the specific bindingbetween anti-Flt1-EBPs and VEGFR1. Furthermore, the anti-Flt1-EBPssuppressed laser-induced choroidal neovascularization in a wet age-relatedmacular degeneration mouse model in vivo. Our results indicate thatanti-Flt1-EBPs as VEGFR1-targeting fusion polypeptides have greatpotential for efficacious anti-angiogenesis to treat retinal-, corneal-,and choroidal neovascularization. | en_US |
dc.description.sponsorship | This work was supported by the National ResearchFoundation (NRF) of Korea funded by the Ministry ofScience and ICT, Korea (2021R1A2C1010682) and theMinistry of Education, Korea (2018R1A6A1A03024231). | en_US |
dc.language | en_US | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.relation.ispartofseries | v. 15, NO 27;32201-32214 | - |
dc.subject | vascular endothelial growth factor receptor 1 targeting peptide | en_US |
dc.subject | lastin-based polypeptides | en_US |
dc.subject | stimuli-responsiveness | en_US |
dc.subject | anti-angiogenesis | en_US |
dc.subject | neovascular diseases | en_US |
dc.title | Vascular Endothelial Growth Factor Receptor 1 Targeting Fusion Polypeptides with Stimuli-Responsiveness for Anti-angiogenesis | en_US |
dc.type | Article | en_US |
dc.relation.no | 27 | - |
dc.relation.volume | 15 | - |
dc.identifier.doi | 10.1021/acsami.3c03989 | en_US |
dc.relation.page | 32201-32214 | - |
dc.relation.journal | ACS APPLIED MATERIALS & INTERFACES | - |
dc.contributor.googleauthor | Kang, Min Jeong | - |
dc.contributor.googleauthor | Roh, Kug-Hwan | - |
dc.contributor.googleauthor | Lee, Jae Sang | - |
dc.contributor.googleauthor | Lee, Jae Hee | - |
dc.contributor.googleauthor | Park, SaeGwang | - |
dc.contributor.googleauthor | Lim, Dong Woo | - |
dc.relation.code | 2023034830 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF BIONANO ENGINEERING | - |
dc.identifier.pid | dlim | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.