Identification and functional characterization of an alternative splice variant within the fourth exon of human nanog
- Title
- Identification and functional characterization of an alternative splice variant within the fourth exon of human nanog
- Author
- 정일엽
- Keywords
- alternative splicing; totipotent stem cells; GATA4 transcription factor; hematopoietic stem cells; NANOG protein human
- Issue Date
- 2005-12
- Publisher
- 생화학분자생물학회
- Citation
- Experimental and Molecular Medicine, v. 37, NO. 6, Page. 601-607
- Abstract
- Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Here, we report the identification of an alternatively-spliced variant of nanog. This variant lacked a stretch of amino acids (residues 168-183) located between the HD and tryptophan-repeat (WR) of the previously-reported full length sequence, suggesting that the deleted sequence functions as a linker and possibly affects the flexibility of the C-terminal transactivation domain relative to the DNA binding domain. Expression of mRNA encoding the splice variant, designated as nanog-delta 48, was much lower than that of the full length version in human ES cells. The ratio of nanog-delta 48 transcript to full length transcript increased, however, in multipotent adult progenitor cells. EMSA analysis revealed that both forms of Nanog were able to bind a nanog binding sequence with roughly the same affinity. A reporter plasmid assay also showed that both variants of nanog modestly repressed transactivation of gata-4, whose expression is proposed to be inhibited by nanog, with comparable potency. We conclude that, despite the difference in primary structure and expression pattern in various stem cells, the alternatively-spliced variant of Nanog has similar activity to that of the full length version.
- URI
- https://www.nature.com/articles/emm200573https://repository.hanyang.ac.kr/handle/20.500.11754/185120
- ISSN
- 1226-3613;2092-6413
- DOI
- 10.1038/emm.2005.73
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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- 8132_정일엽.pdfDownload
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