Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황승용 | - |
dc.date.accessioned | 2023-05-31T01:41:53Z | - |
dc.date.available | 2023-05-31T01:41:53Z | - |
dc.date.issued | 2007-09 | - |
dc.identifier.citation | Journal of Micromechanics and Microengineering, v. 17, NO. 9, Page. 1810-1817 | - |
dc.identifier.issn | 0960-1317;1361-6439 | - |
dc.identifier.uri | https://iopscience.iop.org/article/10.1088/0960-1317/17/9/009 | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/181689 | - |
dc.description.abstract | This paper reports on the development of a low-cost microreactor ( 10 mu l) biochip for DNA polymerase chain reaction ( PCR). The microbiochip ( 20 mm x 28 mm) is a hybrid type that is composed of a polydimethylsiloxane ( PDMS) layer with a serpentine microchannel ( 360 mu m x 100 mu m) chamber and glass substrate integrated with a microheater and thermal microsensor. Because of the hydrophobic chip surface, bubbles are usually created during sample loading in the PMDS-based microchip. These bubbles disrupt the stable biochemical reaction. An improved microreactor chamber was designed using microfluidic simulation. The reactor has a rounded-corner serpentine channel architecture, which enables stable injection into the hydrophobic surface using only a micropipette. The reactor temperature needed for the PCR reaction is controlled within +/- 0.5 degrees C by the LabVIEW software proportional-integrative-derivative (PID) controller. PCR analyses of the sex-determining Y chromosome (SRY) gene and mouse GAPDH gene were successfully performed in less than 54 min by the fabricated microreactor chip. | - |
dc.description.sponsorship | a Korea Research Foundation Grant (KRF-2003-041-D00105). | - |
dc.language | en | - |
dc.publisher | Institute of Physics Publishing | - |
dc.title | PDMS-glass serpentine microchannel chip for time domain PCR with bubble suppression in sample injection | - |
dc.type | Article | - |
dc.relation.no | 9 | - |
dc.relation.volume | 17 | - |
dc.identifier.doi | 10.1088/0960-1317/17/9/009 | - |
dc.relation.page | 1810-1817 | - |
dc.relation.journal | Journal of Micromechanics and Microengineering | - |
dc.contributor.googleauthor | Cho, Chul-Ho | - |
dc.contributor.googleauthor | Cho, Woong | - |
dc.contributor.googleauthor | Ahn, Yoomin | - |
dc.contributor.googleauthor | Hwang, Seung-Yong | - |
dc.sector.campus | E | - |
dc.sector.daehak | 과학기술융합대학 | - |
dc.sector.department | 의약생명과학과 | - |
dc.identifier.pid | syhwang | - |
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