Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황승용 | - |
dc.date.accessioned | 2023-05-31T01:34:00Z | - |
dc.date.available | 2023-05-31T01:34:00Z | - |
dc.date.issued | 2008-08 | - |
dc.identifier.citation | Transactions of the Korean Society of Mechanical Engineers, A, v. 32, NO. 8, Page. 624-632 | - |
dc.identifier.issn | 1226-4873 | - |
dc.identifier.uri | https://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE01028250&language=ko_KR&hasTopBanner=true | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/181679 | - |
dc.description.abstract | In this research, we developed new PDMS-glass based microbiochip consisted of the micromixer and microreactor for DNA ligation. The micromixer was composed of a straight channel integrated with nozzles and pillars, and the microreactor was composed of a serpentine channel. We coated the PDMS chip surface with the 0.25wt.% PVP solution to prevent the bubble generation which was caused by the hydrophobicity of the PDMS. The new micomixer was passive type and the mixing was enhanced by a convective diffusion using the nozzle and pillar. The 10.33mm long micromixer showed the good mixing efficiency of 87.7% at 500 □ 1/min flow rate. We could perform the DNA ligation successfully in the microbiochip, and the ligation time was shortened from 4 hours in conventional laboratory method to 5 min in the microbiochip. | - |
dc.language | ko | - |
dc.subject | DNA ligation | - |
dc.subject | Microbiochip | - |
dc.subject | Micromixer | - |
dc.subject | PVP coating | - |
dc.title | 마이크로 혼합기와 반응기로 구성된 DNA 결찰용 바이오칩에 관한 연구 | - |
dc.title.alternative | A study about biochip combined with micro mixer and reactor for DNA ligation | - |
dc.type | Article | - |
dc.relation.no | 8 | - |
dc.relation.volume | 32 | - |
dc.identifier.doi | 10.3795/KSME-A.2008.32.8.624 | - |
dc.relation.page | 624-632 | - |
dc.relation.journal | Transactions of the Korean Society of Mechanical Engineers, A | - |
dc.contributor.googleauthor | Kang, D.-H. | - |
dc.contributor.googleauthor | Ahn, Y. | - |
dc.contributor.googleauthor | Hwang, S.Y. | - |
dc.sector.campus | E | - |
dc.sector.daehak | 과학기술융합대학 | - |
dc.sector.department | 의약생명과학과 | - |
dc.identifier.pid | syhwang | - |
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