Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황승용 | - |
dc.date.accessioned | 2023-05-31T01:00:45Z | - |
dc.date.available | 2023-05-31T01:00:45Z | - |
dc.date.issued | 2012-09 | - |
dc.identifier.citation | Journal of Asia-Pacific Entomology, v. 15, NO. 3, Page. 389-396 | - |
dc.identifier.issn | 1226-8615;1876-7990 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1226861512000337?via%3Dihub | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/181625 | - |
dc.description.abstract | Proteomic differences between Bemisia tabaci biotypes (B and Q) were investigated by two-dimensional gel electrophoresis in conjunction with mass spectroscopic analysis. Among several protein spots specific to biotype B, carboxylesterase 2 (Coe2) was significantly more expressed in biotype B. Phylogenetic analysis demonstrated the close relationship of Coe2 with Myzus persicae esterase E4. Comparison of full-length cDNA sequences of Coe2 revealed no amino acid differences in functionally important conserved regions between biotypes B and Q. The transcription level of the Coe2 gene (coe2) was 5.8-fold higher in biotype B than in biotype Q but the coe2 copy number was not different between biotypes, suggesting that the overexpression of Coe2 was due to transcriptional up-regulation. Native isoelectric focusing followed by mass spectrophotometric analysis confirmed that the overexpressed pl 5.7 esterase in biotype B was Coe2. In-gel inhibition of Coe2 by three insecticides indicated the interaction of Coe2 with chlorpyrifos-methyl oxon and permethrin, but not with imidacloprid. These findings suggest that overexpression of Coe2 in biotype B can confer chemical defense against pyrethroid and organophosphate insecticides, perhaps by sequestration and hydrolysis, as seen in M. persicae E4. Finally, utility of Coe2 as a potential biotype-specific protein marker is discussed. (C) Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society, 2012. Published by Elsevier B.V. All rights reserved. | - |
dc.description.sponsorship | the Korea Institute of Planning and Evaluation for Technology of Food | - |
dc.language | en | - |
dc.publisher | 한국응용곤충학회 | - |
dc.subject | Bemisia tabaci | - |
dc.subject | Biotypes | - |
dc.subject | Proteomic analysis | - |
dc.subject | Carboxylesterase | - |
dc.subject | Overexpression | - |
dc.title | Proteomics-based identification and characterization of biotype-specific carboxylesterase 2 putatively associated with insecticide resistance in Bemisia tabaci | - |
dc.type | Article | - |
dc.relation.no | 3 | - |
dc.relation.volume | 15 | - |
dc.identifier.doi | 10.1016/j.aspen.2012.03.002 | - |
dc.relation.page | 389-396 | - |
dc.relation.journal | Journal of Asia-Pacific Entomology | - |
dc.contributor.googleauthor | Kang, Soyoung | - |
dc.contributor.googleauthor | Lee, Hye Jung | - |
dc.contributor.googleauthor | Kim, Young Ho | - |
dc.contributor.googleauthor | Kwon, Deok Ho | - |
dc.contributor.googleauthor | Oh, Jung Hun | - |
dc.contributor.googleauthor | Kim, Bum Jun | - |
dc.contributor.googleauthor | Lim, Kook Jin | - |
dc.contributor.googleauthor | Hwang, Seung Yong | - |
dc.contributor.googleauthor | Lee, Seunghwan | - |
dc.contributor.googleauthor | Lee, Si Hyeock | - |
dc.sector.campus | E | - |
dc.sector.daehak | 과학기술융합대학 | - |
dc.sector.department | 의약생명과학과 | - |
dc.identifier.pid | syhwang | - |
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