Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황승용 | - |
dc.date.accessioned | 2023-05-31T00:53:49Z | - |
dc.date.available | 2023-05-31T00:53:49Z | - |
dc.date.issued | 2013-06 | - |
dc.identifier.citation | BioChip Journal, v. 7, NO. 2, Page. 164-172 | - |
dc.identifier.issn | 1976-0280;2092-7843 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs13206-013-7210-z | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/181617 | - |
dc.description.abstract | A protease is any enzyme that catalyzes the hydrolysis of proteins into smaller peptide fragments and amino acids, a process known as proteolysis. They are involved in a multitude of normal biological processes as well as in diseases, including cancer, stroke and infections. Here we present a microfluidic-based assay system to detect proteolytic activity using fluorescence resonance energy transfer (FRET) by quantum dot (QD)-peptide conjugates immobilized on microbeads. As an energy donor, QD was immobilized on the microbead surface by the avidin-biotin interaction. As an energy acceptor, the fluorophore-labeled peptide was then associated with QD, thus quenching the photoluminescence (PL) of the QD. The functionalized microbeads were introduced into the microbiochip and captured by a micropillar in the reaction chamber. In the presence of matrix metalloprotease-7 (MMP-7) as a model protease, the PL of QD quenched by fluorophore was recovered due to the proteolytic activity of MMP-7 in the fabricated microbiochip. Moreover, the FRET efficiency induced by MMP-7 was linearly dependent on the logarithmic concentration of MMP-7. This technology is not limited to sensing MMP-7, but could be used to monitor other protease activities (Schematic diagram). | - |
dc.description.sponsorship | the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (20090074380, 20110005094). | - |
dc.language | en | - |
dc.publisher | 한국바이오칩학회 | - |
dc.subject | Microbiochip | - |
dc.subject | Matrix Metalloproteinase 7 | - |
dc.subject | Fluorescence Resonance Energy Transfer (FRET) | - |
dc.subject | Quantum Dot (QD) | - |
dc.subject | Bead-based assay | - |
dc.subject | Enzyme assay | - |
dc.title | Development of microbiochip for detection of metalloproteinase 7 using fluorescence resonance energy transfer | - |
dc.type | Article | - |
dc.relation.no | 2 | - |
dc.relation.volume | 7 | - |
dc.identifier.doi | 10.1007/s13206-013-7210-z | - |
dc.relation.page | 164-172 | - |
dc.relation.journal | BioChip Journal | - |
dc.contributor.googleauthor | Lee, Seung Yong | - |
dc.contributor.googleauthor | Han, Byoungwook | - |
dc.contributor.googleauthor | Park, Chansoo | - |
dc.contributor.googleauthor | Jeong, Je-Sik | - |
dc.contributor.googleauthor | Ahn, Jeong Jin | - |
dc.contributor.googleauthor | Ha, Seung-Mo | - |
dc.contributor.googleauthor | Hwang, Seung Yong | - |
dc.contributor.googleauthor | Ahn, Yoomin | - |
dc.sector.campus | E | - |
dc.sector.daehak | 과학기술융합대학 | - |
dc.sector.department | 의약생명과학과 | - |
dc.identifier.pid | syhwang | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.