Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황승용 | - |
dc.date.accessioned | 2023-05-26T05:08:46Z | - |
dc.date.available | 2023-05-26T05:08:46Z | - |
dc.date.issued | 2022-06 | - |
dc.identifier.citation | Journal of Virological Methods, v. 304, article no. 114513, Page. 1-4 | - |
dc.identifier.issn | 0166-0934;1879-0984 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S016609342200060X?via%3Dihub | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/181551 | - |
dc.description.abstract | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is associated with high mortality and infectivity rates in humans since its emergence. Analysis using high-accuracy real-time polymerase chain reaction (PCR) is recommended for the detection of general respiratory viruses including SARS-CoV-2, but it takes a long time (e.g. ~ 6 h); moreover, on-site diagnosis is difficult owing to the need for skilled technicians and advanced laboratory facilities. Currently, the importance of point-of-care testing (POCT) is being emphasized for the rapid detection of SARS-CoV-2. Here, we developed a multiplex real-time reverse transcription PCR (rRT-PCR) analysis that not only detects SARS-CoV-2 but also D614G strains with higher contagiousness than wild types among SARS-CoV-2 mutants using probe-based rRT-PCR. Moreover, this method was applied to portable PCR equipment capable of POCT to confirm high detection sensitivity and specificity. Multiple assays were possible with fluorescence labeling of individual probes. Furthermore, using a microfluidic chip-based point-of-care testing rRT-PCR platform, detection time was reduced by more than half compared with the commonly used detection system. This demonstrates that our assay has 100% of high sensitivity and specificity and could thus aid in the rapid and simple screening of SARS-CoV-2 carrying the mutation. We present a rapid detection method for mutations in SARS-CoV-2. | - |
dc.description.sponsorship | Acknowledgment Funding: This work was supported by the Korea Health Industry Development Institute (KHIDI) through the Infectious Disease Preven-tion and Treatment Technology Development Program, funded by Ministry of Health and Welfare (MOHW) [grant number HI20C0061] . | - |
dc.language | en | - |
dc.publisher | Elsevier BV | - |
dc.subject | Real-time PCR | - |
dc.subject | COVID-19 | - |
dc.subject | Molecular diagnosis | - |
dc.subject | SARS-CoV-2 | - |
dc.subject | Spike D614G mutation | - |
dc.subject | Point-of-care | - |
dc.title | Simultaneous detection of SARS-CoV-2 and identification of spike D614G mutation using point-of-care real-time polymerase chain reaction | - |
dc.type | Article | - |
dc.relation.volume | 304 | - |
dc.identifier.doi | 10.1016/j.jviromet.2022.114513 | - |
dc.relation.page | 1-4 | - |
dc.relation.journal | Journal of Virological Methods | - |
dc.contributor.googleauthor | Lee, So Yul | - |
dc.contributor.googleauthor | Lee, Ji Su | - |
dc.contributor.googleauthor | Ahn, Jeong Jin | - |
dc.contributor.googleauthor | Kim, Seung Jun | - |
dc.contributor.googleauthor | Sung, Heungsup | - |
dc.contributor.googleauthor | Huh, Jin Won | - |
dc.contributor.googleauthor | Hwang, Seung Yong | - |
dc.sector.campus | E | - |
dc.sector.daehak | 과학기술융합대학 | - |
dc.sector.department | 의약생명과학과 | - |
dc.identifier.pid | syhwang | - |
dc.identifier.article | 114513 | - |
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