Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Jong Wook Hong | - |
dc.date.accessioned | 2023-01-04T01:51:01Z | - |
dc.date.available | 2023-01-04T01:51:01Z | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v. 29, NO. 2, Page. 244-255 | - |
dc.identifier.issn | 1017-7825;1738-8872 | - |
dc.identifier.uri | https://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1810.10057 | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/178656 | - |
dc.description.abstract | Xylose isomerase (XI; E.C.5.3.1.5) catalyzes the isomerization of xylose to xylulose, which can be used to produce bioethanol through fermentation. Therefore, XI has recently gained attention as a key catalyst in the bioenergy industry. Here, we identified, purified, and characterized a XI (PbXI) from the psychrophilic soil microorganism, Paenibacillus sp. R4. Surprisingly, activity assay results showed that PbXI is not a cold-active enzyme, but displays optimal activity at 60 degrees C. We solved the crystal structure of PbXI at 1.94-angstrom resolution to investigate the origin of its thermostability. The PbXI structure shows a (beta/alpha)(8)-barrel fold with tight tetrameric interactions and it has three divalent metal ions (CaI, CaII, and CaIII). Two metal ions (CaI and CaII) located in the active site are known to be involved in the enzymatic reaction. The third metal ion (CaIII), located near the beta 4-alpha 6 loop region, was newly identified and is thought to be important for the stability of PbXI. Compared with previously determined thermostable and mesophilic XI structures, the beta 1-alpha 2 loop structures near the substrate binding pocket of PbXI were remarkably different. Site-directed mutagenesis studies suggested that the flexible beta 1-alpha 2 loop region is essential for PbXI activity. Our findings provide valuable insights that can be applied in protein engineering to generate low-temperature purpose-specific XI enzymes. | - |
dc.description.sponsorship | The authors thank the staff at the X-ray crystallography core facility of the Korea Basic Science Institute (Ochang, Korea) and BL-5C of the Pohang Accelerator Laboratory (Pohang, Korea) for assistance with data collection. This work was supported by the Korea Polar Research Institute (grant no. PE19210 to J.H.L.). Additional support was provided by the Basic Science Research Program 2018R1A2B6005354 through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning of Korea. The authors thank the staff at the X-ray crystallography core facility of the Korea Basic Science Institute (Ochang, Korea) and BL-5C of the Pohang Accelerator Laboratory (Pohang, Korea) for assistance with data collection. This work was supported by the Korea Polar Research Institute (grant no. PE19210 to J.H.L.). Additional support was provided by the Basic Science Research Program 2018R1A2B6005354 through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning of Korea. | - |
dc.language | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | Cold-active protein | - |
dc.subject | crystal structure | - |
dc.subject | Paenibacillus species | - |
dc.subject | xylose isomerase | - |
dc.subject | X-ray crystallography | - |
dc.title | Crystal Structure and Functional Characterization of a Xylose Isomerase (PbXI) from the Psychrophilic Soil Microorganism, Paenibacillus sp. | - |
dc.type | Article | - |
dc.relation.no | 2 | - |
dc.relation.volume | 29 | - |
dc.identifier.doi | 10.4014/jmb.1810.10057 | - |
dc.relation.page | 244-255 | - |
dc.relation.journal | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.contributor.googleauthor | Park, Sun-Ha | - |
dc.contributor.googleauthor | Kwon, Sunghark | - |
dc.contributor.googleauthor | Lee, Chang Woo | - |
dc.contributor.googleauthor | Kim, Chang Min | - |
dc.contributor.googleauthor | Jeong, Chang Sook | - |
dc.contributor.googleauthor | Kim, Kyung-Jin | - |
dc.contributor.googleauthor | Hong, Jong Wook | - |
dc.contributor.googleauthor | Kim, Hak Jun | - |
dc.contributor.googleauthor | Park, Hyun Ho | - |
dc.contributor.googleauthor | Lee, Jun Hyuck | - |
dc.sector.campus | E | - |
dc.sector.daehak | 공학대학 | - |
dc.sector.department | 생명나노공학과 | - |
dc.identifier.pid | jwh | - |
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