Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2022-12-11T23:56:04Z | - |
dc.date.available | 2022-12-11T23:56:04Z | - |
dc.date.issued | 2022-05 | - |
dc.identifier.citation | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, v. 33, NO. 8, Page. 976-994 | en_US |
dc.identifier.issn | 0920-5063;1568-5624 | en_US |
dc.identifier.uri | https://www.tandfonline.com/doi/full/10.1080/09205063.2022.2030657 | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/178148 | - |
dc.description.abstract | Improving the transfection efficiency of non-viral carriers by using cationic polymers is a useful approach to addressing several challenges in gene therapy, such as cellular uptake, endosomal escape, and toxicity. Among the various cationic polymers, polyamidoamine (PAMAM) dendrimers have been widely utilized because of the abundance of terminal functional groups, thereby enabling further functionalization and enhancing DNA condensation and internalization into cells. The combination of various functional groups is required for these PAMAM dendrimer derivatives to function appropriately for gene delivery. Herein, we synthesized PAMAM G2-HRChol by conjugating dipeptide (histidine-arginine) and cholesterol at different ratios (6% or 23%) on the surface of PAMAM dendrimer generation 2 (PAMAM G2). Both PAMAM G2-HRChol 6% and PAMAM G2-HRChol 23% have buffering capacity, leading to improved endosomal escape after entering the cells. PAMAM G2-HRChol 6% and PAMAM G2-HRChol 23% dendrimers were condensed with pDNA to form nano-polyplexes at a weight ratio of 4 (polymer/pDNA). Polyplexes are positively charged, which facilitates cellular uptake. The transfection efficiency of PAMAM G2-HRChol 6% and PAMAM G2-HRChol 23% dendrimers was similar to that of PEI 25 kDa under optimum conditions, and the cytotoxicity was much lower than that of PEI 25 kDa in HeLa cells. In addition, after apoptin gene transfection was performed, cell death ratios of 34.47% and 22.47% were observed for PAMAM G2-HRChol 6% and PAMAM G2-HRChol 23%, respectively. The results show that a suitable amount of cholesterol can improve gene transfection efficiency, and the PAMAM G2-HRChol 6% dendrimer could be a potential gene carrier in HeLa cells. | en_US |
dc.description.sponsorship | This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF2020R1I1A3070280) and by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT) (NRF2018M3A9B5024060 and NRF-2018M3A9B5024068). | en_US |
dc.language | en | en_US |
dc.publisher | TAYLOR & FRANCIS LTD | en_US |
dc.subject | PAMAM dendrimer | en_US |
dc.subject | cholesterol | en_US |
dc.subject | dipeptide | en_US |
dc.subject | gene delivery | en_US |
dc.subject | transfection | en_US |
dc.title | Preparation and characterization of polyamidoamine dendrimers conjugated with cholesteryl-dipeptide as gene carriers in HeLa cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 8 | - |
dc.relation.volume | 33 | - |
dc.identifier.doi | 10.1080/09205063.2022.2030657 | en_US |
dc.relation.page | 976-994 | - |
dc.relation.journal | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | - |
dc.contributor.googleauthor | Choi, Minyoung | - |
dc.contributor.googleauthor | Le Thi Thuy | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.contributor.googleauthor | Choi, Joon Sig | - |
dc.sector.campus | S | - |
dc.sector.daehak | 공과대학 | - |
dc.sector.department | 생명공학과 | - |
dc.identifier.pid | minhyung | - |
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