Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2022-12-09T06:41:57Z | - |
dc.date.available | 2022-12-09T06:41:57Z | - |
dc.date.issued | 2022-07 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, NO. 14, article no. 7918, Page. 1-17 | en_US |
dc.identifier.issn | 1661-6596;1422-0067 | en_US |
dc.identifier.uri | https://www.mdpi.com/1422-0067/23/14/7918 | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/178113 | - |
dc.description.abstract | Wound healing is a complicated cascading process; disequilibrium among reparative processes leads to the formation of pathologic scars. Herein, we explored the role of mortalin in scar formation and its association with the interleukin-1 alpha receptor using in vitro and in vivo models. To investigate the effects of mortalin, we performed an MTT cell viability assay, qRT-PCR, and Western blot analyses, in addition to immunofluorescence and immunoprecipitation studies using cultured fibroblasts. A rat incisional wound model was used to evaluate the effect of a mortalin-specific shRNA (dE1-RGD/GFP/shMot) Ad vector in scar tissue. In vitro, the mortalin-treated human dermal fibroblast displayed a significant increase in proliferation of type I collagen, alpha-smooth muscle actin, transforming growth factor-beta, phospho-Smad2/3-complex, and NF-kappa B levels. Immunofluorescence staining revealed markedly increased mortalin and interleukin-1 alpha receptor protein in keloid tissue compared to those in normal tissue, suggesting that the association between mortalin and IL-1 alpha receptor was responsible for the fibrogenic effect. In vivo, mortalin-specific shRNA-expressing Ad vectors significantly decreased the scar size and type-I-collagen, alpha-SMA, and phospho-Smad2/3-complex expression in rat incisional scar tissue. Thus, dE1-RGD/GEP/shMot can inhibit the TGF-beta/alpha-SMA axis and NF-kappa B signal pathways in scar formation, and blocking endogenous mortalin could be a potential therapeutic target for keloids. | en_US |
dc.language | en | en_US |
dc.publisher | MDPI | en_US |
dc.source | 91013_윤채옥.pdf | - |
dc.subject | scar | en_US |
dc.subject | keloid | en_US |
dc.subject | mortalin | en_US |
dc.subject | adenovirus | en_US |
dc.subject | interleukin-1 alpha receptor | en_US |
dc.subject | fibrogenesis | en_US |
dc.title | Effect of Mortalin on Scar Formation in Human Dermal Fibroblasts and a Rat Incisional Scar Model | en_US |
dc.type | Article | en_US |
dc.relation.no | 14 | - |
dc.relation.volume | 23 | - |
dc.identifier.doi | 10.3390/ijms23147918 | en_US |
dc.relation.page | 1-17 | - |
dc.relation.journal | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.contributor.googleauthor | Jung, Bok Ki | - |
dc.contributor.googleauthor | Roh, Tai Suk | - |
dc.contributor.googleauthor | Roh, Hyun | - |
dc.contributor.googleauthor | Lee, Ju Hee | - |
dc.contributor.googleauthor | Yun, Chae-Ok | - |
dc.contributor.googleauthor | Lee, Won Jai | - |
dc.sector.campus | S | - |
dc.sector.daehak | 공과대학 | - |
dc.sector.department | 생명공학과 | - |
dc.identifier.pid | chaeok | - |
dc.identifier.orcid | https://orcid.org/0000-0002-9466-4531 | - |
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