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Development of Three-Stage Bioaerosol Sampler for Size-Selective Sampling

Title
Development of Three-Stage Bioaerosol Sampler for Size-Selective Sampling
Author
육세진
Keywords
bioaerosol; collection efficiency; cyclone separator; virtual impactor; BioSampler
Issue Date
2022-07
Publisher
ASME
Citation
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, v. 144, NO. 7, article no. 71008, Page. 1-7
Abstract
In this study, a three-stage bio-aerosol sampler with a sampling flow rate of 170 L/min was designed and fabricated for sampling the bio-aerosols released during human breathing and coughing, and its performance was evaluated. The sampler was constructed using a cyclone separator with a cutoff size of 2.5 μm as a preseparator, a multinozzle virtual impactor with a cutoff size of 0.34 μm as an aerosol concentrator, and a Bio-Sampler, which is a commercial product, for collecting bio-aerosols in a collection fluid. The collection efficiency of the sampler was evaluated through simulations and experiments. Only particles with sizes of 0.1-4 μm were selectively collected in the collection fluid. Bacteriophage bio-aerosols were sampled using the developed sampler and ACD-200 Bobcat sampler, which is a commercial product. The amounts of collected bacteriophages were compared using the polymerase chain reaction (PCR) technique. The sampling performance of the developed sampler was similar to that of the ACD-200 Bobcat sampler. Moreover, the developed sampler showed its ability to sample bio-aerosols of a specific size range and collect them directly in a collection fluid for the PCR analysis. Therefore, the developed sampler is expected to be useful for indoor environmental monitoring by effectively sampling the bio-aerosols released indoors during human breathing and coughing.
URI
https://asmedigitalcollection.asme.org/biomechanical/article/144/7/071008/1131184/Development-of-Three-Stage-Bioaerosol-Sampler-forhttps://repository.hanyang.ac.kr/handle/20.500.11754/177805
ISSN
0148-0731;1528-8951
DOI
10.1115/1.4053504
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > MECHANICAL ENGINEERING(기계공학부) > Articles
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