Antiviral effects and possible mechanisms of action of constituents from Brazilian propolis and related compounds
- Title
- Antiviral effects and possible mechanisms of action of constituents from Brazilian propolis and related compounds
- Author
- PERUMALSAMY HARIBALAN
- Keywords
- propolis; human rhinovirus; p-coumaric acid; kaempferol; mechanism of action; natural antiviral agent
- Issue Date
- 2019-12
- Publisher
- TAYLOR & FRANCIS LTD
- Citation
- JOURNAL OF APICULTURAL RESEARCH, v. 59, no. 4, page. 413-425
- Abstract
- Propolis is a well-known medicinal bee hive product amongst others due to its antiviral properties. Human rhinoviruses (HRVs) are recognized to be responsible for more than 50% of common colds worldwide. This study was performed to determine if kaempferol (KF) and p-coumaric acid (p-CA) extracted from Brazilian propolis and another eight previously known compounds of propolis had antiviral activity toward human rhinovirus (HRV)-2, HRV-3, and HRV-4 in HeLa cells and to determine their mechanism of action using sulforhodamine B assay and real-time reverse transcription polymerase chain reaction. Results were compared with those of a positive control ribavirin. Based on IC50 values, KF, quercetin, chrysin, and luteolin were more pronounced in antiviral activity toward HRV-2 (7.3–37.4 μM), HRV-3 (11.9–20.4 μM), and HRV-4 (12.9–24.4 μM) than a drug reference ribavirin (270.1–323.9 μM). The 35 μM KF and 610 μM p-CA did not interact with the HRV-3 particles. KF and p-CA inhibited HRV-3 infection when added during the early stages (0–4 h) after virus inoculation. These constituents remarkably reduced the RNA replication levels of HRV-3 in the HeLa cell cultures. These findings suggest that KF and p-CA may block or reduce the entrance of the viruses into the cells to protect the cells from the virus destruction and abate virus replication, which may play a crucial role in interfering with expressions of intercellular adhesion molecule-1 and interleukin-6. Further studies about the possible applications of propolis-derived materials as potential products to prevent or treat HRV infection are warranted.
- URI
- https://www.tandfonline.com/doi/full/10.1080/00218839.2019.1695715https://repository.hanyang.ac.kr/handle/20.500.11754/176998
- ISSN
- 0021-8839; 2078-6913
- DOI
- 10.1080/00218839.2019.1695715
- Appears in Collections:
- OFFICE OF ACADEMIC AFFAIRS[S](교무처) > Center for Creative Convergence Education(창의융합교육원) > Articles
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