Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오요한 | - |
dc.date.accessioned | 2022-11-04T04:21:01Z | - |
dc.date.available | 2022-11-04T04:21:01Z | - |
dc.date.issued | 2021-02 | - |
dc.identifier.citation | BIOMATERIALS, v. 269, article no. 120222 | en_US |
dc.identifier.issn | 0142-9612; 1878-5905 | en_US |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0142961220304683?via%3Dihub | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/176291 | - |
dc.description.abstract | Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differentiation of pluripotent stem cells (PSCs) and result in significant costs in large-scale manufacturing. Here, we report a novel PSC culture system via wirelessly controllable optical activation of the fibroblast growth factor (FGF) signaling pathway without the need for supplementation of recombinant FGF2 protein, a key molecule for maintaining pluripotency of PSCs. Using a fusion protein between the cytoplasmic region of the FGF receptor-1 and a light-oxygen-voltage domain, we achieved tunable, blue light-dependent activation of FGF signaling in human and porcine PSCs. Our data demonstrate that a highly controllable optical stimulation of the FGF signaling pathway is sufficient for long-term maintenance of PSCs, without the loss of differentiation potential into three germ layers. This culture system will be a cost-effective platform for a large-scale stem cell culture. | en_US |
dc.description.sponsorship | This work was supported by grants from the National Science Foundation (1547515, G.L.), the Maryland Stem Cell Research Funding (MSCRF, G.L.), the National Institutes of Health through R01NS093213, R01AR070751 (G.L.), and the Global Research Development Center Program from the National Research Foundation of Korea (NRF) (2017K1A4A3014959, G.L. & S.-H.H.). This work was also supported by the NRF grants funded by the Korea government (Ministry of Science and ICT) (2019R1F1A1060296, 2019M3A9H1103783, 2020R1A2C1009172; Y.O.). The authors acknowledge the Cytogenetic Core Facility (supported by the Eunice Kennedy Shriver National Institute of Child Health & Human Development of the National Institutes of Health under Award Number U54HD079123). We thank the Developmental Studies Hybridoma Bank for antibodies. | en_US |
dc.language | en | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | Optogenetics; FGF2; FGF signaling; Pluripotent stem cell; Pluripotency | en_US |
dc.title | Novel culture system via wirelessly controllable optical stimulation of the FGF signaling pathway for human and pig pluripotency | en_US |
dc.type | Article | en_US |
dc.relation.volume | 269 | - |
dc.identifier.doi | 10.1016/j.biomaterials.2020.120222 | en_US |
dc.relation.page | 120222-120222 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.contributor.googleauthor | Choi, In Young | - |
dc.contributor.googleauthor | Lim, HoTae | - |
dc.contributor.googleauthor | Huynh, Alex | - |
dc.contributor.googleauthor | Schofield, James | - |
dc.contributor.googleauthor | Cho, Hyeon Jin | - |
dc.contributor.googleauthor | Lee, Hosuk | - |
dc.contributor.googleauthor | Andersen, Peter | - |
dc.contributor.googleauthor | Shin, Joo Heon | - |
dc.contributor.googleauthor | Heo, Won Do | - |
dc.contributor.googleauthor | Oh, Yohan | - |
dc.relation.code | 2021001169 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOMEDICAL SCIENCE | - |
dc.identifier.pid | yoh | - |
dc.identifier.researcherID | C-9107-2013 | - |
dc.identifier.orcid | https://orcid.org/0000-0002-9249-8664 | - |
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