Novel culture system via wirelessly controllable optical stimulation of the FGF signaling pathway for human and pig pluripotency
- Title
- Novel culture system via wirelessly controllable optical stimulation of the FGF signaling pathway for human and pig pluripotency
- Author
- 오요한
- Keywords
- Optogenetics; FGF2; FGF signaling; Pluripotent stem cell; Pluripotency
- Issue Date
- 2021-02
- Publisher
- ELSEVIER SCI LTD
- Citation
- BIOMATERIALS, v. 269, article no. 120222
- Abstract
- Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differentiation of pluripotent stem cells (PSCs) and result in significant costs in large-scale manufacturing. Here, we report a novel PSC culture system via wirelessly controllable optical activation of the fibroblast growth factor (FGF) signaling pathway without the need for supplementation of recombinant FGF2 protein, a key molecule for maintaining pluripotency of PSCs. Using a fusion protein between the cytoplasmic region of the FGF receptor-1 and a light-oxygen-voltage domain, we achieved tunable, blue light-dependent activation of FGF signaling in human and porcine PSCs. Our data demonstrate that a highly controllable optical stimulation of the FGF signaling pathway is sufficient for long-term maintenance of PSCs, without the loss of differentiation potential into three germ layers. This culture system will be a cost-effective platform for a large-scale stem cell culture.
- URI
- https://www.sciencedirect.com/science/article/pii/S0142961220304683?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/176291
- ISSN
- 0142-9612; 1878-5905
- DOI
- 10.1016/j.biomaterials.2020.120222
- Appears in Collections:
- GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > BIOMEDICAL SCIENCE(의생명과학과) > Articles
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