Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 채필석 | - |
dc.date.accessioned | 2022-10-31T00:44:15Z | - |
dc.date.available | 2022-10-31T00:44:15Z | - |
dc.date.issued | 2018-04 | - |
dc.identifier.citation | Organic and Biomolecular Chemistry, v. 16, NO. 14, Page. 2489-2498 | en_US |
dc.identifier.issn | 1477-0520;1477-0539 | en_US |
dc.identifier.uri | https://pubs.rsc.org/en/content/articlelanding/2018/OB/C8OB00270C | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/176107 | - |
dc.description.abstract | Membrane proteins play critical roles in a variety of cellular processes. For a detailed molecular level understanding of their biological functions and roles in disease, it is necessary to extract them from the native membranes. While the amphipathic nature of these bio-macromolecules presents technical challenges, amphiphilic assistants such as detergents serve as useful tools for membrane protein structural and functional studies. Conventional detergents are limited in their ability to maintain the structural integrity of membrane proteins and thus it is essential to develop novel agents with enhanced properties. Here, we designed and characterized a novel class of amphiphiles with vitamin E (i.e., -tocopherol) as the hydrophobic tail group and saccharide units as the hydrophilic head group. Designated vitamin E-based glycosides (VEGs), these agents were evaluated for their ability to solubilize and stabilize a set of membrane proteins. VEG representatives not only conferred markedly enhanced stability to a diverse range of membrane proteins compared to conventional detergents, but VEG-3 also showed notable efficacy toward stabilization and visualization of a membrane protein complex. In addition to hydrophile-lipophile balance (HLB) of detergent molecules, the chain length and molecular geometry of the detergent hydrophobic group seem key factors in determining detergent efficacy for membrane protein (complex) stability. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (grant number 2016R1A2B2011257 to P. S. C., M. E. and L. G.). | en_US |
dc.language | en | en_US |
dc.publisher | Royal Society of Chemistry | en_US |
dc.title | Vitamin E-based glycoside amphiphiles for membrane protein structural studies | en_US |
dc.type | Article | en_US |
dc.relation.no | 14 | - |
dc.relation.volume | 16 | - |
dc.identifier.doi | 10.1039/c8ob00270c | en_US |
dc.relation.page | 2489-2498 | - |
dc.relation.journal | Organic and Biomolecular Chemistry | - |
dc.contributor.googleauthor | Ehsan, Muhammad | - |
dc.contributor.googleauthor | Du, Yang | - |
dc.contributor.googleauthor | Molist, Iago | - |
dc.contributor.googleauthor | Seven, Alpay B. | - |
dc.contributor.googleauthor | Hariharan, Parameswaran | - |
dc.contributor.googleauthor | Mortensen, Jonas S. | - |
dc.contributor.googleauthor | Ghani, Lubna | - |
dc.contributor.googleauthor | Loland, Claus J. | - |
dc.contributor.googleauthor | Skiniotis, Georgios | - |
dc.contributor.googleauthor | Guan, Lan | - |
dc.contributor.googleauthor | Byrne, Bernadette | - |
dc.contributor.googleauthor | Kobilka, Brian K. | - |
dc.contributor.googleauthor | Chae, Pil Seok | - |
dc.sector.campus | E | - |
dc.sector.daehak | 공학대학 | - |
dc.sector.department | 생명나노공학과 | - |
dc.identifier.pid | pchae | - |
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