Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 신정훈 | - |
dc.date.accessioned | 2022-10-28T01:18:46Z | - |
dc.date.available | 2022-10-28T01:18:46Z | - |
dc.date.issued | 2021-02 | - |
dc.identifier.citation | DIABETES & METABOLISM JOURNAL, v. 45, no. 4, Page. 594-605 | en_US |
dc.identifier.issn | 2233-6079; 2233-6087 | en_US |
dc.identifier.uri | https://www.e-dmj.org/journal/view.php?doi=10.4093/dmj.2020.0049 | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/175916 | - |
dc.description.abstract | Background We previously, reported that granulocyte-colony stimulating factor (G-CSF) reduces cardiomyocyte apoptosis in diabetic cardiomyopathy. However, the underlying mechanisms are not yet fully understood. Therefore, we investigated whether the mechanisms underlying of the anti-apoptotic effects of G-CSF were associated with autophagy using a rat model of diabetic cardiomyopathy. Methods Diabetic cardiomyopathy was induced in rats through a high-fat diet combined with low-dose streptozotocin and the rats were then treated with G-CSF for 5 days. Rat H9c2 cardiac cells were cultured under high glucose conditions as an in vitro model of diabetic cardiomyopathy. The extent of apoptosis and protein levels related to autophagy (Beclin-1, microtubule-binding protein light chain 3 [LC3]-II/LC3-I ratio, and P62) were determined for both models. Autophagy determination was performed using an Autophagy Detection kit. Results G-CSF significantly reduced cardiomyocyte apoptosis in the diabetic myocardium in vivo and led to an increase in Beclin-1 level and the LC3-II/LC3-I ratio, and decreased P62 level. Similarly, G-CSF suppressed apoptosis, increased Beclin-1 level and LC3-II/LC3-I ratio, and decreased P62 level in high glucose-induced H9c2 cardiac cells in vitro. These effects of G-CSF were abrogated by 3-methyladenine, an autophagy inhibitor. In addition, G-CSF significantly increased autophagic flux in vitro. Conclusion Our results suggest that the anti-apoptotic effect of G-CSF might be significantly associated with the up-regulation of autophagy in diabetic cardiomyopathy. | en_US |
dc.description.sponsorship | This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2016R1D1A1-B03931479). | en_US |
dc.language.iso | en | en_US |
dc.publisher | KOREAN DIABETES ASSOC | en_US |
dc.subject | Apoptosis; Autophagy; Diabetic cardiomyopathies; Granulocyte colony-stimulating factor; Myocytes, cardiac | en_US |
dc.title | Role of Autophagy in Granulocyte-Colony Stimulating Factor Induced Anti-Apoptotic Effects in Diabetic Cardiomyopathy | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.4093/dmj.2020.0049 | en_US |
dc.relation.page | 1-17 | - |
dc.relation.journal | DIABETES & METABOLISM JOURNAL | - |
dc.contributor.googleauthor | Shen, Guang-Yin | - |
dc.contributor.googleauthor | Shin, Jeong-Hun | - |
dc.contributor.googleauthor | Song, Yi-Sun | - |
dc.contributor.googleauthor | Joo, Hyun-Woo | - |
dc.contributor.googleauthor | Park, In-Hwa | - |
dc.contributor.googleauthor | Seong, Jin-Hee | - |
dc.contributor.googleauthor | Shin, Na-Kyoung | - |
dc.contributor.googleauthor | Lee, A-Hyeon | - |
dc.contributor.googleauthor | Cho, Young Jong | - |
dc.contributor.googleauthor | Lee, Yonggu | - |
dc.relation.code | 2021009289 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | hyapex | - |
dc.identifier.researcherID | T-3322-2017 | - |
dc.identifier.orcid | https://orcid.org/0000-0002-6718-9763 | - |
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