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2D graphene oxide particles induce unwanted loss in pluripotency and trigger early differentiation in human pluripotent stem cells

Title
2D graphene oxide particles induce unwanted loss in pluripotency and trigger early differentiation in human pluripotent stem cells
Author
박희호
Keywords
2D graphene oxide; hiPSC; Cytotoxicity; Pluripotency; Unwanted differentiation; Early triggering of differentiation
Issue Date
2021-02
Publisher
ELSEVIER
Citation
JOURNAL OF HAZARDOUS MATERIALS, v. 414, article no. 125472
Abstract
The potential health hazards of particulates, such as micro/nano-sized plastics and carbon materials have recently received extensive attention. However, their toxicological properties in association with stem cell differentiation is still relatively unexplored. In this study, we elucidated the cytotoxic effects of 2D graphene oxide (GO), in relation to differentiation of human induced pluripotent stem cells (hiPSCs). Supplementation of GO to hiPSCs demonstrated uptake of GO through the plasma membrane and intracellular accumulation was observed. Increasing the concentration of GO led to reduced viability and increased likelihood of hiPSC colony detachment. Moreover, treatment of GO resulted in significant loss in pluripotency markers, OCT-4 and NANOG. In particular, when hiPSCs were cultured with GO in cardiomyocyte induction medium, upregulation of cardiomyocyte marker, NKX2.5, along with observation of early triggering of differentiation were observed. Taken together, our results highlight the risk in the uptake and accumulation of GO on the stem cell development by unwanted loss in pluripotency and accelerated initiation of differentiation.
URI
https://www.sciencedirect.com/science/article/pii/S0304389421004350?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/175846
ISSN
0304-3894; 1873-3336
DOI
10.1016/j.jhazmat.2021.125472
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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