Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신흥수 | - |
dc.date.accessioned | 2022-10-17T05:34:31Z | - |
dc.date.available | 2022-10-17T05:34:31Z | - |
dc.date.issued | 2021-01 | - |
dc.identifier.citation | BIOFABRICATION, v. 13, no. 1, article no. 015011, page. 1-20 | en_US |
dc.identifier.issn | 1758-5082; 1758-5090 | en_US |
dc.identifier.uri | https://iopscience.iop.org/article/10.1088/1758-5090/abc39c | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/175461 | - |
dc.description.abstract | Three-dimensional (3D)-printed scaffolds have proved to be effective tools for delivering growth factors and cells in bone-tissue engineering. However, delivering spheroids that enhance cellular function remains challenging because the spheroids tend to suffer from low viability, which limits bone regeneration in vivo. Here, we describe a 3D-printed polycaprolactone micro-chamber that can deliver human adipose-derived stem cell spheroids. An in vitro culture of cells from spheroids in the micro-chamber exhibited greater viability and proliferation compared with cells cultured without the chamber. We coated the surface of the chamber with 500 ng of platelet-derived growth factors (PDGFs), and immobilized 50 ng of bone morphogenetic protein 2 (BMP-2) on fragmented fibers, which were incorporated within the spheroids as a new platform for a dual-growth-factor delivery system. The PDGF detached from the chamber within 8 h and the remains were retained on the surface of chamber while the BMP-2 was entrapped by the spheroid. In vitro osteogenic differentiation of the cells from the spheroids in the micro-chamber with dual growth factors enhanced alkaline phosphatase and collagen type 1A expression by factors of 126.7 +/- 19.6 and 89.7 +/- 0.3, respectively, compared with expression in a micro-chamber with no growth factors. In vivo transplantation of the chambers with dual growth factors into mouse calvarial defects resulted in a 77.0 +/- 15.9% of regenerated bone area, while the chamber without growth factors and a defect-only group achieved 7.6 +/- 3.9% and 5.0 +/- 1.9% of regenerated bone areas, respectively. These findings indicate that a spheroid-loaded micro-chamber supplied with dual growth factors can serve as an effective protein-delivery platform that increases stem-cell functioning and bone regeneration. | en_US |
dc.description.sponsorship | This research was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (Grant Nos. 2019R1A2C2084965, 2020M3H4A1A02084829, and 2020R1A4A3078645). | en_US |
dc.language.iso | en | en_US |
dc.publisher | IOP PUBLISHING LTD | en_US |
dc.subject | 3D printing; dual growth factor delivery; osteoinductive spheroid; bone regeneration; micro-chamber | en_US |
dc.title | 3D printed micro-chambers carrying stem cell spheroids and pro-proliferative growth factors for bone tissue regeneration | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 13 | - |
dc.identifier.doi | 10.1088/1758-5090/abc39c | en_US |
dc.relation.page | 1-20 | - |
dc.relation.journal | BIOFABRICATION | - |
dc.contributor.googleauthor | Lee, Jinkyu | - |
dc.contributor.googleauthor | Seok, Ji Min | - |
dc.contributor.googleauthor | Huh, Seung Jae | - |
dc.contributor.googleauthor | Byun, Hayeon | - |
dc.contributor.googleauthor | Lee, Sangmin | - |
dc.contributor.googleauthor | Park, Su A. | - |
dc.contributor.googleauthor | Shin, Heungsoo | - |
dc.relation.code | 2021004791 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | hshin | - |
dc.identifier.orcid | https://orcid.org/0000-0002-9036-155X | - |
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