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3D printed micro-chambers carrying stem cell spheroids and pro-proliferative growth factors for bone tissue regeneration

Title
3D printed micro-chambers carrying stem cell spheroids and pro-proliferative growth factors for bone tissue regeneration
Author
신흥수
Keywords
3D printing; dual growth factor delivery; osteoinductive spheroid; bone regeneration; micro-chamber
Issue Date
2021-01
Publisher
IOP PUBLISHING LTD
Citation
BIOFABRICATION, v. 13, no. 1, article no. 015011, page. 1-20
Abstract
Three-dimensional (3D)-printed scaffolds have proved to be effective tools for delivering growth factors and cells in bone-tissue engineering. However, delivering spheroids that enhance cellular function remains challenging because the spheroids tend to suffer from low viability, which limits bone regeneration in vivo. Here, we describe a 3D-printed polycaprolactone micro-chamber that can deliver human adipose-derived stem cell spheroids. An in vitro culture of cells from spheroids in the micro-chamber exhibited greater viability and proliferation compared with cells cultured without the chamber. We coated the surface of the chamber with 500 ng of platelet-derived growth factors (PDGFs), and immobilized 50 ng of bone morphogenetic protein 2 (BMP-2) on fragmented fibers, which were incorporated within the spheroids as a new platform for a dual-growth-factor delivery system. The PDGF detached from the chamber within 8 h and the remains were retained on the surface of chamber while the BMP-2 was entrapped by the spheroid. In vitro osteogenic differentiation of the cells from the spheroids in the micro-chamber with dual growth factors enhanced alkaline phosphatase and collagen type 1A expression by factors of 126.7 +/- 19.6 and 89.7 +/- 0.3, respectively, compared with expression in a micro-chamber with no growth factors. In vivo transplantation of the chambers with dual growth factors into mouse calvarial defects resulted in a 77.0 +/- 15.9% of regenerated bone area, while the chamber without growth factors and a defect-only group achieved 7.6 +/- 3.9% and 5.0 +/- 1.9% of regenerated bone areas, respectively. These findings indicate that a spheroid-loaded micro-chamber supplied with dual growth factors can serve as an effective protein-delivery platform that increases stem-cell functioning and bone regeneration.
URI
https://iopscience.iop.org/article/10.1088/1758-5090/abc39chttps://repository.hanyang.ac.kr/handle/20.500.11754/175461
ISSN
1758-5082; 1758-5090
DOI
10.1088/1758-5090/abc39c
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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