Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 장광석 | - |
dc.date.accessioned | 2022-10-12T00:11:52Z | - |
dc.date.available | 2022-10-12T00:11:52Z | - |
dc.date.issued | 2021-01 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, NO 2, article no. 480, Page. 1-11 | en_US |
dc.identifier.issn | 1422-0067 | en_US |
dc.identifier.uri | https://www.mdpi.com/1422-0067/22/2/480 | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/175221 | - |
dc.description.abstract | To date, Chlorella vulgaris is the most used species of microalgae in the food and feed additive industries, and also considered as a feasible cell factory for bioproducts. However, the lack of an efficient genetic engineering tool makes it difficult to improve the physiological characteristics of this species. Therefore, the development of new strategic approaches such as genome editing is trying to overcome this hurdle in many research groups. In this study, the possibility of editing the genome of C. vulgaris UTEX395 using clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) has been proven to target nitrate reductase (NR) and adenine phosphoribosyltransferase (APT). Genome-edited mutants, nr and apt, were generated by a DNA-mediated and/or ribonucleoprotein (RNP)-mediated CRISPR-Cas9 system, and isolated based on the negative selection against potassium chlorate or 2-fluoroadenine in place of antibiotics. The mutation of edited genes was demonstrated by the expression level of the correspondent proteins or the mutation of transcripts, and through growth analysis under specific nutrient conditions. In conclusion, this study offers relevant empirical evidence of the possibility of genome editing in C. vulgaris UTEX395 by CRISPR-Cas9 and the practical methods. Additionally, among the generated mutants, nr can provide an easier screening strategy during DNA transformation than the use of antibiotics owing to their auxotrophic characteristics. These results will be a cornerstone for further advancement of the genetics of C. vulgaris. | en_US |
dc.description.sponsorship | This research was supported by the Basic Science Research Program (NRF-2020R1A2C2011998) and “Carbon to X Project” (2020M3H7A1098294) of the National Research Foundation (NRF) of Korea, funded by the Korean government (MSIT), and also the basic Science Research Program funded by the Ministry of Education (2020R1I1A1A01066461). | en_US |
dc.language.iso | en | en_US |
dc.publisher | MDPI | en_US |
dc.subject | Chlorella vulgaris; genome editing; CRISPR-CAS9; nitrate reductase; adenine phosphoribosyltransferase; auxotrophic strain | en_US |
dc.title | Establishment of a Genome Editing Tool Using CRISPR-Cas9 in Chlorella vulgaris UTEX395 | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 22 | - |
dc.identifier.doi | 10.3390/ijms22020480 | en_US |
dc.relation.page | 1-11 | - |
dc.relation.journal | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.contributor.googleauthor | Kim, Jongrae | - |
dc.contributor.googleauthor | Chang, Kwang Suk | - |
dc.contributor.googleauthor | Lee, Sangmuk | - |
dc.contributor.googleauthor | Jin, EonSeon | - |
dc.relation.code | 2021004380 | - |
dc.sector.campus | S | - |
dc.sector.daehak | RESEARCH INSTITUTE[S] | - |
dc.sector.department | THE RESEARCH INSTITUTE FOR NATURAL SCIENCES | - |
dc.identifier.pid | kschang | - |
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