Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이은혜 | - |
dc.date.accessioned | 2022-09-05T00:55:54Z | - |
dc.date.available | 2022-09-05T00:55:54Z | - |
dc.date.issued | 2020-11 | - |
dc.identifier.citation | ANALYTICAL BIOCHEMISTRY, v. 609, article no. 113921, page. 1-9 | en_US |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.issn | 1096-0309 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S000326972030453X?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/172744 | - |
dc.description.abstract | Alzheimer's disease (AD) causes cognitive impairment and serious social isolation. However, there are no effective treatments and even no established confirmatory diagnostic tools for the disease. Amyloid beta (A beta) aggregation in the brain is the best-known pathognomonic mechanism of AD, so various methods for A beta detection have been developed for the diagnosis of this disease. We synthesized two novel, ultra-sensitive peptide probes specialized in detecting A beta aggregates, and examined their potential for future diagnostic application. The peptides are produced through phage high-throughput screening (HTS) and amplified through a serial process called biopanning, which is a repeating method of elution and amplification of probes. We picked phages specific for amyloid from two kinds of phage display. The synthesized peptides were confirmed to have excellent binding affinity to A beta aggregates, by immunohistochemical staining and western blotting using the brains of 3X transgenic (Tg) AD mice at different stages (5-7, 12-17 months old) of AD severity. In the present study, it was confirmed that newly developed amyloid-binding peptides could be used as novel probes for the detection of A beta aggregates, which can be used for clinical diagnosis of AD in the future. | en_US |
dc.description.sponsorship | This research was supported by the Basic Science Research Program of the National Research Foundation of Korea, which is funded by the Ministry of Science, ICT and Future Planning (2018R1A2A2A15023219). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | en_US |
dc.subject | Alzheimer's disease | en_US |
dc.subject | Aβ42 | en_US |
dc.subject | Phage display | en_US |
dc.subject | Peptide aptamer | en_US |
dc.subject | Probe screening | en_US |
dc.subject | Early diagnosis | en_US |
dc.title | Development of peptide aptamers as alternatives for antibody in the detection of amyloid-beta 42 aggregates | en_US |
dc.type | Article | en_US |
dc.relation.volume | 609 | - |
dc.identifier.doi | 10.1016/j.ab.2020.113921 | - |
dc.relation.page | 1-9 | - |
dc.relation.journal | ANALYTICAL BIOCHEMISTRY | - |
dc.contributor.googleauthor | Kim, Sang-Heon | - |
dc.contributor.googleauthor | Lee, Eun-Hye | - |
dc.contributor.googleauthor | Lee, Sang-Choon | - |
dc.contributor.googleauthor | Kim, A-Ru | - |
dc.contributor.googleauthor | Park, Hyun-Hee | - |
dc.contributor.googleauthor | Son, Jeong-Woo | - |
dc.contributor.googleauthor | Koh, Seong-Ho | - |
dc.contributor.googleauthor | Yoon, Moon-Young | - |
dc.relation.code | 2020046863 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF TRANSLATIONAL MEDICAL SCIENCE | - |
dc.identifier.pid | leh23 | - |
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