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Development of peptide aptamers as alternatives for antibody in the detection of amyloid-beta 42 aggregates

Title
Development of peptide aptamers as alternatives for antibody in the detection of amyloid-beta 42 aggregates
Author
이은혜
Keywords
Alzheimer's disease; Aβ42; Phage display; Peptide aptamer; Probe screening; Early diagnosis
Issue Date
2020-11
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
ANALYTICAL BIOCHEMISTRY, v. 609, article no. 113921, page. 1-9
Abstract
Alzheimer's disease (AD) causes cognitive impairment and serious social isolation. However, there are no effective treatments and even no established confirmatory diagnostic tools for the disease. Amyloid beta (A beta) aggregation in the brain is the best-known pathognomonic mechanism of AD, so various methods for A beta detection have been developed for the diagnosis of this disease. We synthesized two novel, ultra-sensitive peptide probes specialized in detecting A beta aggregates, and examined their potential for future diagnostic application. The peptides are produced through phage high-throughput screening (HTS) and amplified through a serial process called biopanning, which is a repeating method of elution and amplification of probes. We picked phages specific for amyloid from two kinds of phage display. The synthesized peptides were confirmed to have excellent binding affinity to A beta aggregates, by immunohistochemical staining and western blotting using the brains of 3X transgenic (Tg) AD mice at different stages (5-7, 12-17 months old) of AD severity. In the present study, it was confirmed that newly developed amyloid-binding peptides could be used as novel probes for the detection of A beta aggregates, which can be used for clinical diagnosis of AD in the future.
URI
https://www.sciencedirect.com/science/article/pii/S000326972030453X?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/172744
ISSN
0003-2697; 1096-0309
DOI
10.1016/j.ab.2020.113921
Appears in Collections:
GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > TRANSLATIONAL MEDICAL SCIENCE(임상의과학과) > Articles
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