Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신흥수 | - |
dc.date.accessioned | 2022-08-03T04:44:13Z | - |
dc.date.available | 2022-08-03T04:44:13Z | - |
dc.date.issued | 2020-10 | - |
dc.identifier.citation | BIOMATERIALS, v. 255, article no. 120192, page. 1-16 | en_US |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.issn | 1878-5905 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0142961220304385?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/172031 | - |
dc.description.abstract | Stem cells with mineralized materials have been used for bone regeneration; however, engineering the complex vascularized structure of the natural bone remains a challenge. Here, we developed platelet-derived growth factor (PDGF) and bio-mineral coated fibers which were then assembled with human adipose-derived stem cells (hADSCs) to form spheroids as building blocks for vascularized bone regeneration. The PDGF incorporated within the spheroid increased the proliferation of hADSCs, which was characterized by Ki-67 staining and DNA contents. Furthermore, the PDGF enhanced not only osteogenic differentiation, but also endothelial differentiation of hADSCs; the cells within the spheroids showed significantly greater gene expression by 2.46 ± 0.14 fold for osteocalcin (OCN) and by 12.85 ± 3.36 fold for von Willebrand factor (vWF) than those without PDGF. Finally, at two months following transplantation of PDGF-incorporated spheroids onto in vivo mouse calvarial defect, the regenerated bone area (42.48 ± 10.84%) was significantly enhanced and the greatest number of capillaries and arterioles with indication of transplanted hADSCs were observed. Moreover, millimeter-scale in vitro tissue prepared by fused assembly of the spheroids exhibited greater mRNA expression-associated to endothelial lineage. Taken together, these findings indicate that stem cell spheroids incorporating PDGF and bio-minerals could be used as a module for successful vascularized bone regeneration. | en_US |
dc.description.sponsorship | This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (Grant No. NRF-2018M3C1B7021994 and 2019R1A2C2084965). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | Fragmented fibers | en_US |
dc.subject | Spheroid | en_US |
dc.subject | hADSCs | en_US |
dc.subject | Stem cell | en_US |
dc.subject | Angiogenic factor | en_US |
dc.subject | Bone regeneration | en_US |
dc.title | Human adipose-derived stem cell spheroids incorporating platelet-derived growth factor (PDGF) and bio-minerals for vascularized bone tissue engineering | en_US |
dc.type | Article | en_US |
dc.relation.volume | 255 | - |
dc.identifier.doi | 10.1016/j.biomaterials.2020.120192 | - |
dc.relation.page | 1-16 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.contributor.googleauthor | Lee, Jinkyu | - |
dc.contributor.googleauthor | Lee, Sangmin | - |
dc.contributor.googleauthor | Ahmad, Taufiq | - |
dc.contributor.googleauthor | Perikamana, Sajeesh Kumar Madhurakkat | - |
dc.contributor.googleauthor | Lee, Jinki | - |
dc.contributor.googleauthor | Kim, Eun Mi | - |
dc.contributor.googleauthor | Shin, Heungsoo | - |
dc.relation.code | 2020048418 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | hshin | - |
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