Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김용희 | - |
dc.date.accessioned | 2022-07-28T01:18:03Z | - |
dc.date.available | 2022-07-28T01:18:03Z | - |
dc.date.issued | 2020-10 | - |
dc.identifier.citation | DRUG DELIVERY, v. 27, no. 1, page. 1501-1513 | en_US |
dc.identifier.issn | 1071-7544 | - |
dc.identifier.issn | 1521-0464 | - |
dc.identifier.uri | https://www.tandfonline.com/doi/full/10.1080/10717544.2020.1837293 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/171850 | - |
dc.description.abstract | In this study, a system for oral delivery of etoposide (ETP) was designed to avoid the problems associated with low and variable bioavailability of a commercially available ETP emulsion comprised of polyethylene glycol, glycerol, and citric acid anhydrous. ETP was complexed with low-molecular-weight methylcellulose (ETP/LMC) and loaded into a water-in-oil-in-water multiple nanoemulsion to formulate an ETP/LMC-nanoemulsion (ELNE). To further enhance the oral bioavailability, an ionic complex formed by anionic lipid 1,2-didecanoyl-sn-glycero-3-phosphate (sodium salt) and cationic Nα-deoxycholyl-l-lysyl-methylester was incorporated into ELNE, yielding ELNE#7. As expected, ELNE#7 showed 4.07- and 2.25-fold increases in artificial membrane and Caco-2/HT29-MTX-E12 permeability (Papp), respectively, resulting in 224% greater oral bioavailability compared with the commercially available ETP emulsion. In contrast, inhibition of clathrin- and caveola-mediated endocytosis, macropinocytosis, and bile acid transporters by chlorpromazine, genistein, amiloride, and actinomycin D in Caco-2/HT-29-MTX-E12 monolayers reduced the Papp by 45.0%, 20.5%, 28.8%, and 31.1%, respectively. These findings suggest that these routes play important roles in enhancing the oral absorption of ELNE#7. In addition, our mechanistic study suggested that P-glycoprotein did not have an inhibitory effect on the permeation of ELNE#7. Notably, ELNE#7 showed significantly enhanced toxicity in LLC and A549 cells compared with ETP-E. These observations support the improved oral absorption of ETP in ELNE#7, suggesting that it is a better alternative than ETP emulsion. | en_US |
dc.description.sponsorship | This research was supported by the Basic Research Program through the National Research Foundation of Korea (NRF), funded by the Korean Government Ministry of Science and ICT [MSIT; Grant No. NRF-2020R1F1A1069889]. | en_US |
dc.language.iso | en | en_US |
dc.publisher | TAYLOR & FRANCIS LTD | en_US |
dc.subject | Etoposide | en_US |
dc.subject | nanoemulsion | en_US |
dc.subject | permeability | en_US |
dc.subject | oral bioavailability | en_US |
dc.subject | bile acid transporter-mediated uptake | en_US |
dc.subject | oral absorption | en_US |
dc.title | Enhanced oral bioavailability of an etoposide multiple nanoemulsion incorporating a deoxycholic acid derivative-lipid complex | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 27 | - |
dc.identifier.doi | 10.1080/10717544.2020.1837293 | - |
dc.relation.page | 1501-1513 | - |
dc.relation.journal | DRUG DELIVERY | - |
dc.contributor.googleauthor | Jha, Saurav Kumar | - |
dc.contributor.googleauthor | Han, Hee-Soo | - |
dc.contributor.googleauthor | Subedi, Laxman | - |
dc.contributor.googleauthor | Pangeni, Rudra | - |
dc.contributor.googleauthor | Chung, Jee Young | - |
dc.contributor.googleauthor | Kweon, Seho | - |
dc.contributor.googleauthor | Choi, Jeong Uk | - |
dc.contributor.googleauthor | Byun, Youngro | - |
dc.contributor.googleauthor | Kim, Yong-Hee | - |
dc.contributor.googleauthor | Park, Jin Woo | - |
dc.relation.code | 2020047380 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | yongheekim | - |
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