Multi-Functional MPT Protein as a Therapeutic Agent against Mycobacterium tuberculosis
- Title
- Multi-Functional MPT Protein as a Therapeutic Agent against Mycobacterium tuberculosis
- Author
- 김재성
- Keywords
- Mycobacterium tuberculosis; MPT peptide; TBK1; p47phox; hexokinase 2; macrophages
- Issue Date
- 2021-05
- Publisher
- MDPI
- Citation
- BIOMEDICINES, v. 9, NO 5, Page. 545-564
- Abstract
- Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis (TB), avoids the host immune system through its virulence factors. MPT63 and MPT64 are the virulence factors secreted by MTB which regulate host proteins for the survival and proliferation of MTB in the host. Here, we found that MPT63 bound directly with TBK1 and p47phox, whereas MPT64 interacted with TBK1 and HK2. We constructed a MPT63/64-derived multifunctional recombinant protein (rMPT) that was able to interact with TBK1, p47phox, or HK2. rMPT was shown to regulate IFN-beta levels and increase inflammation and concentration of reactive oxygen species (ROS), while targeting macrophages and killing MTB, both in vitro and in vivo. Furthermore, the identification of the role of rMPT against MTB was achieved via vaccination in a mouse model. Taken together, we here present rMPT, which, by regulating important immune signaling systems, can be considered an effective vaccine or therapeutic agent against MTB.
- URI
- https://www.proquest.com/docview/2532325924?accountid=11283https://repository.hanyang.ac.kr/handle/20.500.11754/171713
- ISSN
- 22279059
- DOI
- 10.3390/biomedicines9050545
- Appears in Collections:
- RESEARCH INSTITUTE[E](부설연구소) > INSTITUTE OF NATURAL SCIENCE AND TECHNOLOGY(이학기술연구소) > Articles
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