Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이은규 | - |
dc.date.accessioned | 2022-05-30T07:32:49Z | - |
dc.date.available | 2022-05-30T07:32:49Z | - |
dc.date.issued | 1999-12 | - |
dc.identifier.citation | KSBB Journal. 2000-01 14(6):651-654 | en_US |
dc.identifier.issn | 1225-7117 | - |
dc.identifier.issn | 2288-8268 | - |
dc.identifier.uri | https://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE02247367 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/171231 | - |
dc.description.abstract | Using recombinant human growth hormone as a model protein, we carried out unfolding by adding a denaturant such as urea, guanidine HCl, or SDS followed by refolding by dilution and dialysis. The objectives were to monitor the structural changes during in vitro refolding process and, based on the results, to develop a quantitative method of refolding progress assessment. The changes in surface hydrophobicity were measured by fluorescence tagging of 1-anilinonaphthalene-8-sulfonate(1,8-ANS) to the hydrophobic portions, and those in the secondary structure were monitored by using far UV-CD(circular dichroism) spectroscopy. Also, we used RP-HPLC to separate and quantify the folded and unfolded proteins to correlate the result with the structure analysis. Our results indicate the surface hydrophobicity are well correlated with the formations of the secondary structure, primarily α-helices, as well as the disulfide bridges. We expect this monitoring techique can be applied in industrial fields as a means to quantitatively assess the progress of in-vitro refolding of recombinant proteins. | en_US |
dc.description.sponsorship | 본 연구는 1998년도 학술진흥재단 과학기술기초 중점연구과제(과제번호 1998-020-E00075) 연구비 지원으로 수행되었으며 이에 감사드립니다. 본 연구에 참여한 조태훈은 교육부지정 국책대학원인 한양대학교 신소재공정공학원의 장학지원에 대하여 감사의 뜻을 전합니다. | en_US |
dc.language.iso | ko_KR | en_US |
dc.publisher | 한국생물공학회 | en_US |
dc.subject | unfolding | en_US |
dc.subject | refolding | en_US |
dc.subject | 1,8-ANS | en_US |
dc.subject | hydrophobicity | en_US |
dc.subject | α-helices | en_US |
dc.title | 재조합 인성장호르몬의 in vitro 풀림과 재접힘 과정의 구조변화 모니터링 | en_US |
dc.title.alternative | Monitoring of Structural Changes during in vitro Unfolding and Refolding of Recombinant Human Growth Hormone | en_US |
dc.type | Article | en_US |
dc.relation.journal | 한국생물공학회지(KOREAN JOURNAL OF BIOTECHNOLOGY AND BIOENGINEERING) | - |
dc.contributor.googleauthor | 조태훈 | - |
dc.contributor.googleauthor | 채영규 | - |
dc.contributor.googleauthor | 안상점 | - |
dc.contributor.googleauthor | 이은규 | - |
dc.relation.code | 2012101518 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF BIONANO ENGINEERING | - |
dc.identifier.pid | eklee | - |
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