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dc.contributor.author원정임-
dc.date.accessioned2022-05-11T05:55:16Z-
dc.date.available2022-05-11T05:55:16Z-
dc.date.issued2020-09-
dc.identifier.citationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, no. 18, article no. 6452en_US
dc.identifier.issn1422-0067-
dc.identifier.urihttps://www.mdpi.com/1422-0067/21/18/6452-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/170759-
dc.description.abstractRNA decay is an important regulatory mechanism for gene expression at the posttranscriptional level. Although the main pathways and major enzymes that facilitate this process are well defined, global analysis of RNA turnover remains under-investigated. Recent advances in the application of next-generation sequencing technology enable its use in order to examine various RNA decay patterns at the genome-wide scale. In this study, we investigated human RNA decay patterns using parallel analysis of RNA end-sequencing (PARE-seq) data fromXRN1-knockdown HeLa cell lines, followed by a comparison of steady state and degraded mRNA levels from RNA-seq and PARE-seq data, respectively. The results revealed 1103 and 1347 transcripts classified as stable and unstable candidates, respectively. Of the unstable candidates, we found that a subset of thereplication-dependent histonetranscripts was polyadenylated and rapidly degraded. Additionally, we identified 380 endonucleolytically cleaved candidates by analyzing the most abundant PARE sequence on a transcript. Of these, 41.4% of genes were classified as unstable genes, which implied that their endonucleolytic cleavage might affect their mRNA stability. Furthermore, we identified 1877 decapped candidates, includingHSP90B1andSWI5,having the most abundant PARE sequences at the 5 '-end positions of the transcripts. These results provide a useful resource for further analysis of RNA decay patterns in human cells.en_US
dc.description.sponsorshipThis work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT, No. 2020R1A2C100730311 to J.-I.W.) and by the National Research Foundation of Korea (NRF) grants funded by the Korea government (NRF-2020R1F1A1050128 to J.Y. and NRF-2 2018R1D1A1B07049751 to D.-H.J.). This work was also supported by a grant from the Next-Generation BioGreen 21 Program (Project No. PJ01366801), Rural Development Administration, Republic of Korea to D.-H.J.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectmRNA decayen_US
dc.subjectparallel analysis of RNA endsen_US
dc.subjectXRN1en_US
dc.subjectdecappingen_US
dc.titleGlobal Analysis of the Human RNA Degradome Reveals Widespread Decapped and Endonucleolytic Cleaved Transcriptsen_US
dc.typeArticleen_US
dc.relation.no18-
dc.relation.volume21-
dc.identifier.doi10.3390/ijms21186452-
dc.relation.page6452-6467-
dc.relation.journalINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES-
dc.contributor.googleauthorWon, Jung-Im-
dc.contributor.googleauthorShin, JaeMoon-
dc.contributor.googleauthorPark, So Young-
dc.contributor.googleauthorYoon, JeeHee-
dc.contributor.googleauthorJeong, Dong-Hoon-
dc.relation.code2020050347-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentINNOVATION CENTER FOR ENGINEERING EDUCATION-
dc.identifier.pidjiwon-


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