Knockout of slms10 gene (Solyc02g079810) encoding bhlh transcription factor using crispr/cas9 system confers male sterility phenotype in tomato
- Title
- Knockout of slms10 gene (Solyc02g079810) encoding bhlh transcription factor using crispr/cas9 system confers male sterility phenotype in tomato
- Author
- 배상수
- Keywords
- CRISPR/Cas9; bHLH transcription factor; male sterility; SlMS10 gene; tomatoes
- Issue Date
- 2020-09
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Citation
- Plants, v. 9, no. 9, article no. 1189
- Abstract
- The utilization of male sterility into hybrid seed production reduces its cost and ensures high purity of tomato varieties because it does not produce pollen and has exserted stigmas. Here, we report on the generation of gene edited lines into male sterility phenotype by knockout ofSlMS10gene (Solyc02g079810)encoding the bHLH transcription factor that regulates meiosis and cell death of the tapetum during microsporogenesis in the tomato. Twenty-eight gene edited lines out of 60 transgenic plants were selected. Of these, eleven different mutation types at the target site of theSlMS10gene were selected through deep sequencing analysis. These mutations were confirmed to be transmitted to subsequent generations. The lines without the transferred DNA (T-DNA) were obtained by segregation in the T(1)and T(2)generations. In addition, we showed that the cr-ms10-1-4 mutant line exhibited dysfunctional meiosis and abnormal tapetum during flower development, resulting in no pollen production. RT-PCR analysis showed that the most genes associated with pollen and tapetum development in tomatoes had lower expression in the cr-ms10-1-4 mutant line compared to wild type. We demonstrate that modification of theSlMS10gene via CRISPR/Cas9-mediated genome editing results in male sterility of tomato plants. Our results suggest an alternative approach to generating male sterility in crops.
- URI
- https://www.mdpi.com/2223-7747/9/9/1189https://repository.hanyang.ac.kr/handle/20.500.11754/170667
- ISSN
- 2223-7747
- DOI
- 10.3390/plants9091189
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
- Files in This Item:
- Knockout of slms10 gene (Solyc02g079810) encoding bhlh transcription factor using crispr cas9 system confers male sterility phenotype in tomato.pdfDownload
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