Socheongryongtang Modulates Asthma-Related Changes via Modulation of TNF-α and T-bet as well as IFN-γ in an Asthma Murine Model

Abstract

In 2017 the World Health Organization (WHO) reported that 235 million people suffered from asthma, and that 383,000 deaths were due to asthma in 2015. Asthma cannot be completely eradicated and the medications for asthma are associated with many adverse effects. Socheongryongtang is one of the prescriptions which has traditionally been used for the treatment of pulmonary disease, but the anti-asthmatic mechanism is unclear. To investigate the anti-asthmatic mechanism of socheongryongtang, BALB/c mice were divided into five groups: control, asthma-induced control, dexamethasone treatment, and 150 mg/kg or 1500 mg/kg socheongryongtang treatment and several biomarkers were analyzed, such as white blood cell (WBC) and differential counts in broncheoalveolar fluid (BALF), immunoglobulin E (IgE) in serum, and morphological changes/helper T cell-related cytokines/transcription factor in the lung. The therapeutic ingredients were also analyzed. Socheongryongtang inhibited the neutrophils differentiation in BALF, controlled interleukin (IL)-12p40 releasing, down-regulated not only GATA-3 and helper 2 T (Th2) cell transcription factors but also IL-4, and also decreased the level of tumor necrosis factor (TNF)-alpha in the lung. In addition, through high-performance liquid chromatography (HPLC) analysis, we confirmed that the therapeutic ingredients in socheongryongtang were paeoniflorin, liquiritin, and glycyrrhizin. The oral intake of 7.3 g of socheongryongtang is beneficial for suppressing the possibility of the occurrence of asthma via modulation of TNF-alpha and T-bet as well as IFN-gamma.