Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조석현 | - |
dc.date.accessioned | 2022-03-29T05:45:59Z | - |
dc.date.available | 2022-03-29T05:45:59Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | JOURNAL OF CLINICAL MEDICINE, v. 9, no. 7, article no. 2128 | en_US |
dc.identifier.issn | 2077-0383 | - |
dc.identifier.uri | https://www.mdpi.com/2077-0383/9/7/2128 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/169495 | - |
dc.description.abstract | Parkinson's disease (PD) is the most common neurodegenerative movement disorder, characterized by olfactory dysfunction in the early stages. alpha-Synuclein pathologies in the olfactory organs are shown to spread to the brain through the nose-brain axis. We first developed a nasal epithelial PD cellular model by treating RPMI-2650 cells with alpha-synuclein preformed fibrils (PFF). Upon uptake of PFF, RPMI-2650 cells showed mitochondrial proteome alteration and downregulation of parkin, which has previously been identified as a nasal biomarker of PD. Functional cluster analysis of differentially expressed genes in RPMI-2650 cells revealed various pathways affected by alpha-synuclein pathology, including the detection of chemical stimulus involved in sensory perception, olfactory receptor activity, and sensory perception of smell. Among genes that were most affected, we validated, by real-time quantitative PCR, the downregulation ofMAP3K8,OR10A4,GRM2,OR51B6, andOR9A2,as well as upregulation ofIFIT1B,EPN1, OR1D5, LCN, and OTOL1in PFF-treated RPMI-2650 cells. Subsequent analyses of clinical samples showed a downregulation ofOR10A4andOR9A2transcripts and an upregulation ofIFIT1Bin cells isolated from the nasal fluid of PD patients, as compared to those from the controls (cutoff value = 0.5689 forOR9A2,with 72.4% sensitivity and 75% specificity, and 1.4658 forIFIT1B,with 81.8% sensitivity and 77.8% specificity). Expression levels of these nasal PD markers were not altered in nasal fluid cells from SWEDD (scans without evidence of dopaminergic deficits) patients with PD-like motor symptoms. These nasal markers were significantly altered in patients of PD with hyposmia compared to the control hyposmic subjects. Our results validated the alpha-synuclein-treated nasal epithelial cell model to identify novel biomarkers for PD and suggest the utility of olfactory transcripts, along with olfactory dysfunction, in the diagnosis of PD. | en_US |
dc.description.sponsorship | This research was supported by grants (2017M3C7A1043848 and 2017R1D1A1B03028797) from the National Research Foundation of Korea funded by the Korean Ministry of Science, ICT & Future Planning (MSIP). | en_US |
dc.language.iso | en | en_US |
dc.publisher | MDPI | en_US |
dc.subject | nasal epithelial cell model | en_US |
dc.subject | Parkinson's disease | en_US |
dc.subject | RPMI-2650 cells | en_US |
dc.subject | olfactory biomarker | en_US |
dc.subject | alpha-synuclein preformed fibril | en_US |
dc.subject | olfactory dysfunction | en_US |
dc.title | Novel Nasal Epithelial Cell Markers of Parkinson's Disease Identified Using Cells Treated with alpha-Synuclein Preformed Fibrils | en_US |
dc.type | Article | en_US |
dc.relation.no | 7 | - |
dc.relation.volume | 9 | - |
dc.identifier.doi | 10.3390/jcm9072128 | - |
dc.relation.page | 1-15 | - |
dc.relation.journal | JOURNAL OF CLINICAL MEDICINE | - |
dc.contributor.googleauthor | Kim, Hyojung | - |
dc.contributor.googleauthor | Kang, Seok-Jae | - |
dc.contributor.googleauthor | Jo, Young Mi | - |
dc.contributor.googleauthor | Park, Sanggyu | - |
dc.contributor.googleauthor | Yun, Seung Pil | - |
dc.contributor.googleauthor | Kim, Hee-Tae | - |
dc.contributor.googleauthor | Lee, Nae-Eung | - |
dc.contributor.googleauthor | Kim, Yong-Sang | - |
dc.contributor.googleauthor | Cho, Seok Hyun | - |
dc.contributor.googleauthor | Lee, Yunjong | - |
dc.relation.code | 2020047025 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | shcho | - |
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