Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2022-03-29T02:00:26Z | - |
dc.date.available | 2022-03-29T02:00:26Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | NANOSCALE, v. 12, no. 25, page. 13606-13617 | en_US |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.issn | 2040-3372 | - |
dc.identifier.uri | https://pubs.rsc.org/en/content/articlelanding/2020/NR/D0NR01367F | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/169487 | - |
dc.description.abstract | Acute lung injury (ALI) is a severe inflammatory lung disease. A high mobility group box-1 (HMGB-1) derived RAGE-antagonist peptide (RAP) was previously developed for anti-inflammatory therapy for ALI. Due to its specific binding to RAGE on the surface of inflammatory cells, the RAP may facilitate polymer-mediated intracellular delivery of plasmid DNA (pDNA) into the inflammatory cells. To evaluate this hypothesis, a pDNA/polymer/RAP ternary-complex was produced and applied for ALI gene therapy. Dexamethasone-conjugated polyamidoamine G2 (PAM-D) was used as a gene carrier, and the adiponectin (APN) gene was employed as a therapeutic gene. First, the ratio of pDNA to PAM-D was optimized in terms of anti-inflammation and low toxicity. Then, the RAP was added to the pDNA/PAM-D complex, producing the pDNA/PAM-D/RAP complex. The transfection efficiency of the luciferase plasmid (pLuc)/PAM-D/RAP reached its maximum at a weight ratio of 1 : 2 : 9. At this weight ratio, pLuc/PAM-D/RAP had a higher transfection efficiency than pLuc/PAM-D or pLuc/RAP. The transfection efficiency of pLuc/PAM-D/RAP decreased due to competition with free RAPs, suggesting the RAGE-mediated endocytosis of the complex. In the LPS-activated ALI mouse models, intratracheal administration of APN plasmid (pAPN)/PAM-D/RAP induced higher APN expression and less pro-inflammatory cytokines in the lungs of ALI animal models than pAPN/PEI25k, pAPN/RAP, and pAPN/PAM-D. Hematoxylin and eosin staining confirmed the higher anti-inflammatory effect of pAPN/PAM-D/RAP than the other complexes in the ALI models. Therefore, RAP-mediated enhanced delivery of pAPN/PAM-D may be useful for the development of a treatment for ALI. | en_US |
dc.description.sponsorship | This work was supported by a grant from the Ministry of Health and Welfare in Korea (HI18C1236). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ROYAL SOC CHEMISTRY | en_US |
dc.subject | HEME OXYGENASE-1 GENE | en_US |
dc.subject | DEXAMETHASONE | en_US |
dc.subject | CARRIER | en_US |
dc.subject | LIPOPOLYSACCHARIDE | en_US |
dc.subject | POLYETHYLENIMINE | en_US |
dc.subject | LIPOPOLYMER | en_US |
dc.subject | ACTIVATION | en_US |
dc.subject | DENDRIMER | en_US |
dc.subject | VECTORS | en_US |
dc.subject | MODELS | en_US |
dc.title | A RAGE-antagonist peptide potentiates polymeric micelle-mediated intracellular delivery of plasmid DNA for acute lung injury gene therapy | en_US |
dc.type | Article | en_US |
dc.relation.no | 25 | - |
dc.relation.volume | 12 | - |
dc.identifier.doi | 10.1039/d0nr01367f | - |
dc.relation.page | 13606-13617 | - |
dc.relation.journal | NANOSCALE | - |
dc.contributor.googleauthor | Piao, Chunxian | - |
dc.contributor.googleauthor | Zhuang, Chuanyu | - |
dc.contributor.googleauthor | Choi, Myoungjee | - |
dc.contributor.googleauthor | Ha, Junkyu | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.relation.code | 2020054450 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
dc.identifier.orcid | https://orcid.org/0000-0002-7083-9296 | - |
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