Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박장환 | - |
dc.date.accessioned | 2022-03-23T02:22:20Z | - |
dc.date.available | 2022-03-23T02:22:20Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | STEM CELL REPORTS, v. 15, no. 1, page. 171-184 | en_US |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S2213671120301776?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/169341 | - |
dc.description.abstract | Human pluripotent stem cells (PSCs)through somatic cell nuclear transfer (SCNT) may be an important source for regenerative medicine. The low derivation efficiency of stem cells and the accessibility of human oocytes are the main obstacles to their application. We previously reported that the efficiency of SCNT was increased by overexpression of H3K9me3 demethylase. Here, we applied a modified derivation method to the PSC line and first obtained human SCNT-PSC lines derived from both donated cryo-preserved oocytes and cord blood cells with a homozygous human leukocyte antigen (HLA) type. The SCNT-PSCs have very similar characteristics with embryonic stem cells (ESCs) and additionally have shown immunocompatibility in an in vitro and in vivo humanized mouse with a matching HLA type. Our study demonstrates that SCNT technology using donated cryopreserved oocytes and cord blood cells with a known HLA type provides a promising method for establishing a human HLA-matched SCNT-PSC bank for regenerative medicine. | en_US |
dc.description.sponsorship | We thank Drs. Jung-Il Lee and Seung-Young Go for technical help for humanized mouse project. This research is supported by the Bio & Medical Technology Development Program (2015M3A9C6028961, 2017M3A9F8072235, and 2017M3A 9C6061284) and Basic Science Research Program (2019 R1A2C2005681) of NRF and MSICT of Republic of Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | CELL PRESS | en_US |
dc.subject | somatic cell nuclear transfer | en_US |
dc.subject | frozen oocytes | en_US |
dc.subject | frozen cord blood cells | en_US |
dc.subject | homozygous HLA | en_US |
dc.subject | pluripotent stem cells | en_US |
dc.title | Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 15 | - |
dc.identifier.doi | 10.1016/j.stemcr.2020.05.005 | - |
dc.relation.page | 171-184 | - |
dc.relation.journal | STEM CELL REPORTS | - |
dc.contributor.googleauthor | Lee, Jeoung Eun | - |
dc.contributor.googleauthor | Lee, Ji Yoon | - |
dc.contributor.googleauthor | Park, Chang-Hwan | - |
dc.contributor.googleauthor | Eum, Jin Hee | - |
dc.contributor.googleauthor | Jung, Soo Kyung | - |
dc.contributor.googleauthor | Han, A-Reum | - |
dc.contributor.googleauthor | Seol, Dong-Won | - |
dc.contributor.googleauthor | Lee, Jin Saem | - |
dc.contributor.googleauthor | Shin, Hyun Soo | - |
dc.contributor.googleauthor | Im, Jung Ho | - |
dc.relation.code | 2020051529 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOMEDICAL SCIENCE | - |
dc.identifier.pid | chshpark | - |
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