Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 허준호 | - |
dc.date.accessioned | 2022-03-22T07:51:20Z | - |
dc.date.available | 2022-03-22T07:51:20Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | NATURE COMMUNICATIONS, v. 11, no. 1, article no. 3596 | en_US |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://www.nature.com/articles/s41467-020-17418-8 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/169318 | - |
dc.description.abstract | CRISPR effectors, which comprise a CRISPR-Cas protein and a guide (g)RNA derived from the bacterial immune system, are widely used for target-specific genome editing. When the gRNA recognizes genomic loci with sequences that are similar to the target, deleterious mutations can occur. Off-target mutations with a frequency below 0.5% remain mostly undetected by current genome-wide off-target detection techniques. Here we report a method to effectively detect extremely small amounts of mutated DNA based on predicted off-target-specific amplification. In this study, we used various genome editors to induce intracellular genome mutations, and the CRISPR amplification method detected off-target mutations at a significantly higher rate (1.6 similar to 984 fold increase) than an existing targeted amplicon sequencing method. In the near future, CRISPR amplification in combination with genome-wide off-target detection methods will allow detection of genome editor-induced off-target mutations with high sensitivity and in a non-biased manner. | en_US |
dc.description.sponsorship | We thank Dr. Kyu-Tae Chang for helpful discussions. This research was supported by grants from the National Research Foundation funded by the Korean Ministry of Education, Science and Technology (NRF-2019R1C1C1006603, NRF-2017R1E1A1A01074529, NRF-2018M3A9H3021707, NRF-2019M3A9H110378), the Technology Innovation Program funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea) (20009707), and KRIBB Research Initiative Program (KGM1052021, KGM4252021, KGM5382012). | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | NEXT-GENERATION | en_US |
dc.subject | WEB TOOL | en_US |
dc.subject | NUCLEASES | en_US |
dc.subject | CPF1 | en_US |
dc.subject | SEQ | en_US |
dc.subject | MUTAGENESIS | en_US |
dc.subject | CLEAVAGE | en_US |
dc.subject | CHOPCHOP | en_US |
dc.subject | RARE | en_US |
dc.title | Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 11 | - |
dc.identifier.doi | 10.1038/s41467-020-17418-8 | - |
dc.relation.page | 3596-3596 | - |
dc.relation.journal | NATURE COMMUNICATIONS | - |
dc.contributor.googleauthor | Kang, Seung-Hun | - |
dc.contributor.googleauthor | Lee, Wi-jae | - |
dc.contributor.googleauthor | An, Ju-Hyun | - |
dc.contributor.googleauthor | Lee, Jong-Hee | - |
dc.contributor.googleauthor | Kim, Young-Hyun | - |
dc.contributor.googleauthor | Kim, Hanseop | - |
dc.contributor.googleauthor | Oh, Yeounsun | - |
dc.contributor.googleauthor | Park, Young-Ho | - |
dc.contributor.googleauthor | Jin, Yeung Bae | - |
dc.contributor.googleauthor | Hur, Junho K. | - |
dc.relation.code | 2020046258 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | juhur | - |
dc.identifier.researcherID | ADK-0757-2022 | - |
dc.identifier.orcid | https://orcid.org/0000-0003-3794-1149 | - |
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