Cell-based electrochemical cytosensor for rapid and sensitive evaluation of the anticancer effects of saponin on human malignant melanoma cells
- Title
- Cell-based electrochemical cytosensor for rapid and sensitive evaluation of the anticancer effects of saponin on human malignant melanoma cells
- Author
- 성기훈
- Keywords
- Cell-based biosensor; Cytosensor; Electrochemical detection; Human skin malignant melanoma; Saponin; Anticancer drug assessment
- Issue Date
- 2021-08
- Publisher
- ELSEVIER SCIENCE SA
- Citation
- BIOELECTROCHEMISTRY, v. 140, Page. 107813-107821
- Abstract
- Discovering new anticancer agents and analyzing their activities is a vital part of drug development, but it requires a huge amount of time and resources, leading to the increasing demands for more-effective techniques. Herein, a novel and simple cell-based electrochemical biosensor, referred to as a cytosensor, was proposed to investigate the electrochemical behavior of human skin malignant melanoma (SK-MEL28) cells and the anticancer effect of saponin on cell viability. To enhance both electrocatalytic properties and biocompatibility, gold nanoparticles were electrochemically deposited onto a conductive substrate, and poly-L-lysine was further added to the electrode surface. Electric signals from SK-MEL28 cells on the electrodes were obtained from cyclic voltammetry and differential pulse voltammetry. The cathodic peak current was proportional to the cell viability and showed a detection range of 2,880–40,000 cells per device with an excellent linear cell number-intensity relationship (). Furthermore, the anticancer effect of saponin on SK-MEL28 cells was clearly established at concentrations higher than 20 , which was highly consistent with conventional assays. Moreover, the developed electrochemical cytosensor for evaluating anticancer effects enabled rapid (<2 min), sensitive (LOQ: 2,880 cells/device), and non-invasive measurements, thus providing a new avenue for assessing the anticancer drugs in vitro.
- URI
- https://www.sciencedirect.com/science/article/pii/S1567539421000761https://repository.hanyang.ac.kr/handle/20.500.11754/169000
- ISSN
- 1567-5394
- DOI
- 10.1016/j.bioelechem.2021.107813
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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