Simultaneous Determination of Phenolic Acids and Phthalide Compounds by Liquid Chromatography for Quality Assessment of Rhizoma Cnidii

Abstract

This paper describes a simple, rapid, and validated reversed-phase high-performance liquid chromatographic method developed for the determination of 4 major bioactive constituents, namely, chlorogenic acid, ferulic acid, senkyunolide A, and Z-ligustilide in Rhizoma Cnidii extract. A Capcell Pak C-18 chromatographic column (150 x 4.6 mm, 3 mu m) was used with mobile phases consisting of 0.1% formic acid, acetonitrile, and methanol at a flow rate of 0.8 mL/min and UV detection at 285 nm. Comprehensive validation of the method included evaluation of linearity, repeatability, recovery, and stability. Excellent linear behavior (r(2) ˃0.99) was observed over the concentration range of 2-100 mu g/mL for the compounds under investigation. Repeatability and accuracy were evaluated by intra- and interday assays