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A single-cell survey of Drosophila blood

Title
A single-cell survey of Drosophila blood
Author
남진우
Keywords
IMMUNE-RESPONSE; HEMATOPOIETIC STEM; INNATE IMMUNITY; RIBOSOMAL-RNA; CYCLE EXIT; DIFFERENTIATION; GENE; EXPRESSION; HEMOCYTES; REVEALS
Issue Date
2020-05
Publisher
ELIFE SCIENCES PUBLICATIONS LTD
Citation
ELIFE, v. 9, article no. e54818
Abstract
Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) to map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes into different states based on the expression of genes involved in cell cycle, antimicrobial response, and metabolism together with the identification of intermediate states. Further, we discovered rare subsets within crystal cells and lamellocytes that express fibroblast growth factor (FGF) ligand branchless and receptor breathless, respectively. We demonstrate that these FGF components are required for mediating effective immune responses against parasitoid wasp eggs, highlighting a novel role for FGF signaling in inter-hemocyte crosstalk. Our scRNA-seq analysis reveals the diversity of hemocytes and provides a rich resource of gene expression profiles for a systems-level understanding of their functions.
URI
https://elifesciences.org/articles/54818https://repository.hanyang.ac.kr/handle/20.500.11754/166411
ISSN
2050-084X
DOI
10.7554/eLife.54818
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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