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dc.contributor.author남은우-
dc.date.accessioned2021-10-22T07:09:15Z-
dc.date.available2021-10-22T07:09:15Z-
dc.date.issued2020-02-
dc.identifier.citationTRANSFUSION AND APHERESIS SCIENCE, v. 59, no. 1, article no. 102603en_US
dc.identifier.issn1473-0502-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S147305021930134X?via%3Dihub-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/165656-
dc.description.abstractPurposes: We investigated the impact of time, storage temperature, and dimethyl sulfoxide (DMSO) on the viability of HSCs, as well as on apoptotic changes in thawed CB. Materials & Methods: Thirteen units of cryopreserved CB were thawed and half of each sample was stored at room temperature (RT) and the other half at 4℃, without removing or diluting DMSO. Flow cytometry was employed to enumerate total nucleated cells (TNCs), total/viable CD34+ cells, and early/late apoptotic cells using anti-CD45, anti-CD34, and annexin V(AnV), 7-amino actinomycin D(AAD) staining, respectively. Results: In CBs stored at 4℃ there were no significant changes in numbers of TNCs, total/viable CD34+ cells, or early/late apoptotic cell up to 48 h. However, the numbers of these cells declined significantly at RT. Total and viable CD34+ cell counts did not change for up to 6 h at RT but viable CD34+ cells decreased significantly after 24 h, and total CD34+ cell after 48 h. Early and late apoptosis tended to increase with time at RT, and numbers of viable CD34+ cells and early apoptotic cells differed significantly between RT and 4℃ after 48 h. Conclusions: There are no significant changes of viability and apoptosis in CBs stored in DMSO at 4℃ until 48 h after thawing, while at RT, there are no significant changes of total/viable CD34+ cell counts or in the proportion of apoptotic cells for at least 6 h after thawing.en_US
dc.description.sponsorshipThis work was supported by the Research Fund of Hanyang University (HY-2016)en_US
dc.language.isoenen_US
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDen_US
dc.subjectCord blooden_US
dc.subjectCryopreservationen_US
dc.subjectDimethyl sulfoxideen_US
dc.subjectViabilityen_US
dc.subjectApoptosisen_US
dc.titleCryopreserved cord blood mononuclear cells in DMSO are healthy for at least 6 hours after thawingen_US
dc.typeArticleen_US
dc.relation.no1-
dc.relation.volume59-
dc.identifier.doi10.1016/j.transci.2019.06.028-
dc.relation.page1-5-
dc.relation.journalTRANSFUSION AND APHERESIS SCIENCE-
dc.contributor.googleauthorLee, Young-Ho-
dc.contributor.googleauthorKoh, Hani-
dc.contributor.googleauthorNam, Eunwoo-
dc.contributor.googleauthorKim, Yong Joo-
dc.relation.code2020053590-
dc.sector.campusS-
dc.sector.daehakRESEARCH INSTITUTE[S]-
dc.sector.departmentRHEUMATISM CENTER-
dc.identifier.pideunwoonam-
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